Çirci Esra, Özalay Metin, Caylak Berrin, Bacanli Didem, Derincek Alihan, Tuncay I Cengiz
Department of Orthopedics and Traumatology, İstanbul Training and Research Hospital, İstanbul, Turkey.
Acta Orthop Traumatol Turc. 2013;47(3):193-200. doi: 10.3944/aott.2013.2792.
The aim of this study was to examine the effect of oophorectomy in the formation of epidural fibrosis in a rat laminectomy model.
Thirty-six 12-month-old adult female Sprague-Dawley rats were used in this study. Rats were evenly divided into two groups; oophorectomized and sham-operated. Bilateral oophorectomy was performed on the 18 rats in the oophorectomized group. Three weeks after oophorectomy, rats in both groups underwent complete bilateral laminectomy at the L2 and L3 vertebral levels. Rats were divided into 3 equal groups and sacrificed in groups of 4 at the 4th, 8t, and 12th weeks postoperatively and the lumbar spine excised en bloc, fixed and decalcified. Sections were stained with hematoxylin and eosin and Masson's trichrome were used to evaluate epidural fibrosis, acute inflammation, chronic inflammation, and vascular proliferation.
The mean histological sum grade of the epidural fibrosis was greater in the oophorectomized group (p>0.05).
Endogenous estrogen could have an effect on epidural fibrosis formation after lumbar laminectomy in rats.
本研究旨在探讨卵巢切除术对大鼠椎板切除模型中硬膜外纤维化形成的影响。
本研究使用了36只12个月大的成年雌性Sprague-Dawley大鼠。将大鼠平均分为两组；卵巢切除组和假手术组。对卵巢切除组的18只大鼠进行双侧卵巢切除术。卵巢切除术后3周，两组大鼠均在L2和L3椎体水平进行完全双侧椎板切除术。将大鼠分为3个相等的组，并在术后第4、8和12周每组4只处死，将腰椎整块切除，固定并脱钙。切片用苏木精和伊红染色，并用Masson三色染色法评估硬膜外纤维化、急性炎症、慢性炎症和血管增生。
卵巢切除组硬膜外纤维化的平均组织学总评分更高（p>0.05）。
内源性雌激素可能对大鼠腰椎椎板切除术后硬膜外纤维化的形成有影响。
Zotero 插件