Gao Wu-Jun, Li Shu-Fen, Zhang Guo-Jun, Wang Ning-Na, Deng Chuan-Liang, Lu Long-Dou
College of Life Sciences, Henan Normal University, Xijxiang, China.
Biosci Biotechnol Biochem. 2013;77(6):1193-9. doi: 10.1271/bbb.120943. Epub 2013 Jun 7.
To identify rapidly a number of genes probably involved in sex determination and differentiation of the dioecious plant Asparagus officinalis, gene expression profiles in early flower development for male and female plants were investigated by microarray assay with 8,665 probes. In total, 638 male-biased and 543 female-biased genes were identified. These genes with biased-expression for male and female were involved in a variety of processes associated with molecular functions, cellular components, and biological processes, suggesting that a complex mechanism underlies the sex development of asparagus. Among the differentially expressed genes involved in the reproductive process, a number of genes associated with floral development were identified. Reverse transcription-PCR was performed for validation, and the results were largely consistent with those obtained by microarray analysis. The findings of this study might contribute to understanding of the molecular mechanisms of sex determination and differentiation in dioecious asparagus and provide a foundation for further studies of this plant.
为了快速鉴定一些可能参与雌雄异株植物芦笋性别决定和分化的基因，利用含有8665个探针的微阵列分析技术，研究了雄株和雌株在花发育早期的基因表达谱。总共鉴定出638个雄性偏向基因和543个雌性偏向基因。这些在雄性和雌性中表达有偏向性的基因参与了与分子功能、细胞成分和生物学过程相关的多种过程，这表明芦笋的性别发育存在复杂的机制。在参与生殖过程的差异表达基因中，鉴定出了一些与花发育相关的基因。进行了逆转录-聚合酶链反应以进行验证，结果与微阵列分析结果基本一致。本研究结果可能有助于理解雌雄异株芦笋性别决定和分化的分子机制，并为该植物的进一步研究提供基础。
