Enteric Virus Group, Murdoch Childrens Research Institute, Melbourne, Australia.
Inflamm Bowel Dis. 2013 Jul;19(8):1598-608. doi: 10.1097/MIB.0b013e318292477c.
The role of bacteriophage in Crohn's disease (CD) is unknown. This study investigated the abundance of phages in ileal and colonic samples from pediatric CD patients and controls.
Ileal and colonic biopsies from 6 CD patients, gut wash samples from 3 CD patients, and ileal biopsies from 6 noninflammatory bowel disease patients (controls) were analyzed for the presence of bacteriophage using 454 high-throughput pyrosequencing. A sequence-independent single-primer amplification method was used to amplify viral sequences.
A total of 186,143 high quality reads were obtained from the 4 sample populations. Contigs and sequence clusters (generated from unassembled singletons) were aligned with sequences from the National Center for Biotechnology Information viral reference database and analyzed by MEGAN. The largest number of viral hits was obtained from the CD gut wash samples (n = 691), followed by CD ileal samples (n = 52), control ileum samples (n = 20), and CD colonic samples (n = 1). The most abundant virus sequences identified belonged to the Caudovirales phage.
Our study characterized a diverse phage community in the gut of CD patients. In this study, we have identified differences in phage composition between CD patients and control individuals. The large abundance of phages in CD ileum tissue and CD gut wash sample suggests a role of phage in CD development. The role of phage dysbiosis in CD is currently unknown but opens up a new area of research.
噬菌体在克罗恩病（CD）中的作用尚不清楚。本研究调查了儿科 CD 患者和对照者回肠和结肠样本中噬菌体的丰度。
使用 454 高通量焦磷酸测序分析 6 例 CD 患者的回肠和结肠活检、3 例 CD 患者的肠道冲洗样本和 6 例非炎症性肠病患者（对照）的回肠活检中噬菌体的存在情况。使用无序列依赖性单引物扩增方法扩增病毒序列。
从 4 个样本群体中获得了 186143 个高质量读数。将 Contigs 和序列簇（由未组装的单峰生成）与来自国家生物技术信息中心病毒参考数据库的序列对齐，并通过 MEGAN 进行分析。从 CD 肠道冲洗样本中获得了最多的病毒命中数（n = 691），其次是 CD 回肠样本（n = 52）、对照回肠样本（n = 20）和 CD 结肠样本（n = 1）。鉴定出的最丰富的病毒序列属于长尾病毒目噬菌体。
我们的研究描述了 CD 患者肠道中多样化的噬菌体群落。在这项研究中，我们发现了 CD 患者和对照个体之间噬菌体组成的差异。CD 回肠组织和 CD 肠道冲洗样本中噬菌体的大量存在表明噬菌体在 CD 发展中起作用。噬菌体失调在 CD 中的作用尚不清楚，但开辟了一个新的研究领域。
