Moscow State Academy of Veterinary Medicine and Biotechnology, Akad. Skryabin Str. 23, Moscow, 109472, Russia,
Cell Biochem Biophys. 2013;67(3):1365-70. doi: 10.1007/s12013-013-9668-7.
Photoactivated ("caged") fluorescent dyes are modern tools for structure and function studies of cell membranes and subcellular organelles. Recently synthesized precursors of rhodamine fluorescent dyes (abbreviations PFD813 and PFD814) important for microscopic probing of biological objects have been studied in solution. In order to characterize the behavior at interfaces, monolayers of PFD813 and PFD814 on water have been formed and investigated. The interactions of these precursors with the biomembrane component dimyristoylphosphatidylethanolamine in monolayers at the air-water interface and after transfer to glass plates have been studied by measuring monolayer parameters and spectroscopic properties before and after photo-chemical formation of the fluorescent rhodamine dyes Rho813 and Rho814, respectively.
光激活（“笼状”）荧光染料是研究细胞膜和亚细胞细胞器结构和功能的现代工具。最近合成的罗丹明荧光染料（缩写为 PFD813 和 PFD814）前体对于生物对象的微观探测很重要，它们在溶液中已经得到了研究。为了研究在界面上的行为，在水面上形成了 PFD813 和 PFD814 的单层并对其进行了研究。通过测量单层参数和光化学形成荧光罗丹明染料 Rho813 和 Rho814 前后的光谱特性，研究了这些前体在空气-水界面上单分子层中与二肉豆蔻酰磷脂酰乙醇胺生物膜成分的相互作用，以及转移到玻璃片上后的相互作用。
