Wells M A
Biochim Biophys Acta. 1975 Mar 24;380(3):501-5. doi: 10.1016/0005-2760(75)90119-8.
A new, high yield, procedure for the purification of the phospholipases A2 of Crotalus adamanteus venom is described. The procedure involves precipitation of the bulk of venom proteins with 50% isopropanol, precipitation of the enzymes from the isopranol soluble material with neodynium chloride, and final purification on DEAE cellulose. An overall yield of approximately 80% is achieved.
描述了一种用于纯化金刚背响尾蛇毒液中磷脂酶A2的新的高产方法。该方法包括用50%异丙醇沉淀大部分毒液蛋白，用氯化钕从异丙醇可溶物中沉淀酶，以及在DEAE纤维素上进行最终纯化。总体产率约为80%。
