Faculty of Biology, Technion-Israel Institute of Technology, Haifa, Israel.
Environ Microbiol Rep. 2013 Jun;5(3):475-82. doi: 10.1111/1758-2229.12037. Epub 2013 Feb 17.
The Gulf of Aqaba, extending north to the Red Sea, is an oligotrophic basin with typical open ocean gyre characteristics. Here we report on the existence of diverse microbial rhodopsins in the Gulf of Aqaba, based on 454-pyrosequencing-generated metagenome and metatranscriptome data sets, obtained from the microbial fraction smaller than 1.6 μm. Bacterial SAR11, SAR86 and archaeal proteorhodopsins as well as viral-like rhodopsins were detected on the DNA level. On the RNA level, only SAR11 and SAR86 proteorhodopsin transcripts were detected. Our results add to the growing evidence that microbial rhodopsins are a diverse, abundant and widespread protein family.
亚喀巴湾向北延伸至红海，是一个贫营养盆地，具有典型的开阔海洋回旋流特征。在这里，我们根据从小于 1.6μm 的微生物部分获得的 454 焦磷酸测序生成的宏基因组和宏转录组数据集，报告了在亚喀巴湾存在多样化的微生物视紫红质。在 DNA 水平上检测到细菌 SAR11、SAR86 和古菌视紫红质以及类似病毒的视紫红质。在 RNA 水平上，仅检测到 SAR11 和 SAR86 噬菌体型视紫红质转录本。我们的研究结果进一步证明，微生物视紫红质是一个多样化、丰富和广泛存在的蛋白质家族。
