Saharudin Khairul Arifah, Sreekantan Srimala, Abd Aziz Siti Nor Qurratu Aini, Hazan Roshasnorlyza, Lai Chin Wei, Mydin Rabiatul Basria S M N, Mat Ishak
School of Materials and Mineral Resources Engineering, Universiti Sains Malaysia, Engineering Campus, 14300 Nibong Tebal, Seberang Perai Selatan, Pulau Pinang, Malaysia.
J Nanosci Nanotechnol. 2013 Mar;13(3):1696-705. doi: 10.1166/jnn.2013.7115.
The present study deals with surface modification of Ti6Al4V alloy via anodization technique. The morphology, structure, adhesion and bioactivity of Ti6Al4V alloy after anodization process were investigated in detail. The influence of fluoride content and direct circuit (DC) applied voltage during anodization of Ti6Al4V alloy in a bath with electrolytes composed of ethylene glycol (EG) and ammonium fluoride (NH4F) were considered. It was found that the average pore sizes and length of nanoporous or nanotubes were increasing with the fluoride content and applied voltage. A minimum of 3 wt% of NH4F is required to grow a self-organized nanotube arrays. As the fluoride content was increased to 5 wt%, TiO2 nanotubes with average diameter of 110 nm and 3.4 microm lengths were successfully synthesized. It is noteworthy to point out that the rate of the nanotube formation was increasing up to 9 microm thick bioactive TiO2 nanotubes layer as anodization time was increased to 3 h. Based on the results obtained, the PA6 cells cultured on anodic Ti6Al4V alloy showed highest level of cell viability and greater cell adhesion compared to the flat Ti6Al4V foil substrate. In fact, highly ordered nanotubes structure on Ti6Al4V alloy can provide beneficial effects for PA6 cells in attachment and proliferation.
本研究通过阳极氧化技术对Ti6Al4V合金进行表面改性。详细研究了阳极氧化处理后Ti6Al4V合金的形貌、结构、附着力和生物活性。考虑了在由乙二醇（EG）和氟化铵（NH4F）组成的电解液浴中对Ti6Al4V合金进行阳极氧化时氟化物含量和直流（DC）施加电压的影响。结果发现，纳米多孔或纳米管的平均孔径和长度随氟化物含量和施加电压的增加而增大。生长自组织纳米管阵列至少需要3 wt%的NH4F。当氟化物含量增加到5 wt%时，成功合成了平均直径为110 nm、长度为3.4微米的TiO2纳米管。值得指出的是，随着阳极氧化时间增加到3小时，纳米管形成速率增加，直至形成9微米厚的生物活性TiO2纳米管层。基于所得结果，与平整的Ti6Al4V箔基板相比，在阳极氧化的Ti6Al4V合金上培养的PA6细胞显示出最高水平的细胞活力和更强的细胞附着力。事实上，Ti6Al4V合金上高度有序的纳米管结构可为PA6细胞的附着和增殖提供有益影响。
