从牛脑可溶性部分纯化碱性核糖核酸酶

Purification of an alkaline ribonuclease from soluble fraction of beef brain.

作者信息

Okazaki H, Ittel M E, Niedergang C, Mandel P

出版信息

Biochim Biophys Acta. 1975 May 23;391(1):84-95. doi: 10.1016/0005-2744(75)90155-2.

Abstract

A ribonuclease (ribonucleate 3-pyrimidine-oligonucleotidohydrolase, EC 3.1.4.22) was purified 8300-fold from soluble fraction of beef brain and its properties were investigated. The enzyme is an endonuclease capable of hydrolyzing tRNA, rRNA, poly(C), but shows no activity towards poly(U), poly(A), and poly(G). The preparation is free of deoxyribonuclease, non-specific phosphodiesterase and phosphomonoesterase activity. The enzyme has a pH optimum of 7.6, is not heat stable, has a molecular weight of 25 000, and has a K-m of 134 mu rRNA and K-m of 1600 mug poly(C) per ml.

摘要

从牛脑可溶性部分纯化出一种核糖核酸酶（核糖核酸3 - 嘧啶 - 寡核苷酸水解酶，EC 3.1.4.22），纯化倍数达8300倍，并对其性质进行了研究。该酶是一种内切核酸酶，能够水解tRNA、rRNA、聚（C），但对聚（U）、聚（A）和聚（G）无活性。该制剂不含脱氧核糖核酸酶、非特异性磷酸二酯酶和磷酸单酯酶活性。该酶的最适pH为7.6，不耐热，分子量为25000，每毫升对rRNA的K-m为134μg，对聚（C）的K-m为1600μg。