Department of Orthopaedic Surgery, Hadassah-Hebrew University Medical Center, Jerusalem, Israel.
J Reconstr Microsurg. 2013 Oct;29(8):495-500. doi: 10.1055/s-0033-1348032. Epub 2013 Jun 11.
Immune system augmentation, using the antigen glatiramer acetate (GA), which is known to affect cellular immunity, has been shown to have a positive effect on peripheral nerve regeneration. We aimed to compare the effect of GA on the regeneration of crushed versus transected nerves. Wild-type rats underwent crush or transection and repair of the sciatic nerve. They were examined 3 weeks postinjury histologically (axon count) and functionally (tibialis anterior muscle weight and footprint analysis). GA was found to augment regeneration both histologically and functionally. In the transected nerve, a significant increase in axon count distal to the injury site was seen in the GA group versus control. A similar yet statistically insignificant trend was found in the crushed nerve. Improvement was found in the footprint analysis between the GA and control groups in both crush and transected nerve groups. We found improvement in the footprint analysis in the crush versus transection group. GA was found to improve the regeneration of the peripheral nerve. Histologically, this was more pronounced in the transection injury. The discrepancy between the different functional measures examined may be explained by the distance of the reinnervated muscles evaluated from the injury site.
免疫系统增强，使用抗原醋酸格拉替雷（GA），已知其会影响细胞免疫，已被证明对周围神经再生有积极影响。我们旨在比较 GA 对挤压伤与切断伤神经再生的影响。野生型大鼠接受坐骨神经挤压伤或切断伤和修复。伤后 3 周进行组织学（轴突计数）和功能（胫骨前肌重量和足迹分析）检查。GA 在组织学和功能上均增强了再生。在切断伤中，GA 组与对照组相比，损伤部位远端的轴突计数明显增加。在挤压伤中也发现了类似但统计学上无显著差异的趋势。在挤压伤和切断伤两组中，GA 组与对照组在足迹分析中均有改善。与切断伤相比，挤压伤的足迹分析有改善。GA 改善了周围神经的再生。组织学上，在切断伤中更为明显。不同功能测量指标之间的差异可能是由于评估的再支配肌肉与损伤部位的距离不同所致。
