Nahhas F, Barnea E
Department of Obstetrics and Gynecology, Rambam Medical Center, Haifa, Israel.
Am J Reprod Immunol. 1990 Mar-Apr;22(3-4):105-8. doi: 10.1111/j.1600-0897.1990.tb00651.x.
Early pregnancy factor (EPF) is a factor that has been shown to be involved in the maintenance of the fetal allograft in several mammalian species including man. In that capacity it serves as a link between fertilization and immunomodulation. In the present study we have investigated the secretion of EPF by the human conceptus before and after implantation using rosette inhibition antibody concentration (T11 monoclonal antibody). In isolated trophoblastic cells at gestational week 7-9 there was a significant secretion of EPF. For the first 8 days in culture, secretion was maximal; later, however, at days 13 and 21 these levels declined. Further the EPF content of human embryonal extracts was examined. In various organs, spinal cord, liver, and kidney, significant EPF activity was detected. In women undergoing in vitro fertilization/embryo transfer, a total of 52 preimplantational embryos were cultured for 36 hours. No significant EPF activity was detected in the surrounding media. Two of the 11 patients studied became pregnant during the same cycle after embryo transfer. In conclusion, the preimplantational origin of EPF is maternal, while that of the post-implantational embryo is of embryonal origin.
早孕因子（EPF）是一种已被证明在包括人类在内的几种哺乳动物物种中参与维持胎儿同种异体移植的因子。它以这种能力充当受精与免疫调节之间的联系。在本研究中，我们使用玫瑰花结抑制抗体浓度（T11单克隆抗体）研究了植入前后人类孕体分泌EPF的情况。在妊娠第7 - 9周的分离滋养层细胞中，有显著的EPF分泌。在培养的前8天，分泌量最大；然而，在第13天和第21天，这些水平下降。此外，还检测了人类胚胎提取物中的EPF含量。在各种器官，如脊髓、肝脏和肾脏中，检测到了显著的EPF活性。在接受体外受精/胚胎移植的女性中，共将52个植入前胚胎培养了36小时。在周围培养基中未检测到显著的EPF活性。在研究的11名患者中，有2名在胚胎移植后的同一周期内怀孕。总之，植入前EPF的来源是母体，而植入后胚胎的EPF来源是胚胎源性的。
