蛋白酪氨酸激酶 6 与鼻咽癌不良预后和转移相关。
Protein tyrosine kinase 6 is associated with nasopharyngeal carcinoma poor prognosis and metastasis.
机构信息
Department of Oncology, Second Affiliated Hospital of Guangzhou medical college, 250 Changgang Road East, Guangzhou 510260, China.
出版信息
J Transl Med. 2013 Jun 9;11:140. doi: 10.1186/1479-5876-11-140.
BACKGROUND
The aim of this study was to analyze the expression of protein tyrosine kinase 6 (PTK6) in nasopharyngeal carcinoma (NPC) samples, and to identify whether PTK6 can serve as a biomarker for the diagnosis and prognosis of NPC.
METHODS
We used quantitative RT-PCR and Western blotting analysis to detect mRNA and protein expression of PTK6 in NPC cell lines and immortalized nasopharyngeal epithelial cell lines. 31 NPC and 16 non-tumorous nasopharyngeal mucosa biopsies were collected to detect the difference in the expression of mRNA level of PTK6 by quantitative RT-PCR. We also collected 178 NPC and 10 normal nasopharyngeal epithelial cases with clinical follow-up data to investigate the expression of PTK6 by immunohistochemistry staining (IHC). PTK6 overexpression on cell growth and colony formation ability were measured by the method of cell proliferation assay and colony formation assay.
RESULTS
The expression of PTK6 was higher in most of NPC cell lines at both mRNA and protein levels than in immortalized nasopharyngeal epithelial cell lines (NPECs) induced by Bmi-1 (Bmi-1/NPEC1, and Bmi-1/NPEC2). The mRNA level of PTK6 was high in NPC biopsies compared to non-tumorous nasopharyngeal mucosa biopsies. IHC results showed the expression of PTK6 was significantly correlated to tumor size (P<0.001), clinical stage (P<0.001), and metastasis (P=0.016). The patients with high-expression of PTK6 had a significantly poor prognosis compared to those of low-expression (47.8% versus 80.0%, P<0.001), especially in the patients at the advanced stages (42.2% versus 79.1%, P<0.001). Multivariate analysis indicated that the level of PTK6 expression was an independent prognostic factor for the overall survival of patients with NPC (P <0.001). Overexpression of PTK6 in HNE1 cells enhanced the ability of cell proliferation and colony formation.
CONCLUSIONS
Our results suggest that high-expression of PTK6 is an independent factor for NPC patients and it might serve as a potential prognostic biomarker for patients with NPC.
背景
本研究旨在分析蛋白酪氨酸激酶 6（PTK6）在鼻咽癌（NPC）样本中的表达，并确定 PTK6 是否可作为 NPC 诊断和预后的生物标志物。
方法
我们使用定量 RT-PCR 和 Western blot 分析检测 NPC 细胞系和永生化鼻咽上皮细胞系中 PTK6 的 mRNA 和蛋白表达。收集 31 例 NPC 和 16 例非肿瘤性鼻咽黏膜活检组织，通过定量 RT-PCR 检测 PTK6 mRNA 水平的差异。还收集了 178 例 NPC 和 10 例有临床随访资料的正常鼻咽上皮病例，通过免疫组织化学染色（IHC）检测 PTK6 的表达。通过细胞增殖试验和集落形成试验测量 PTK6 过表达对细胞生长和集落形成能力的影响。
结果
在大多数 NPC 细胞系中，PTK6 的表达在 mRNA 和蛋白水平上均高于由 Bmi-1 诱导的永生化鼻咽上皮细胞系（Bmi-1/NPEC1 和 Bmi-1/NPEC2）。与非肿瘤性鼻咽黏膜活检组织相比，NPC 活检组织中 PTK6 的 mRNA 水平较高。IHC 结果表明，PTK6 的表达与肿瘤大小（P<0.001）、临床分期（P<0.001）和转移（P=0.016）显著相关。与低表达相比，PTK6 高表达的患者预后明显较差（47.8% 比 80.0%，P<0.001），尤其是在晚期患者中（42.2% 比 79.1%，P<0.001）。多因素分析表明，PTK6 表达水平是 NPC 患者总生存期的独立预后因素（P<0.001）。在 HNE1 细胞中过表达 PTK6 增强了细胞增殖和集落形成的能力。
结论
我们的研究结果表明，PTK6 的高表达是 NPC 患者的独立因素，它可能是 NPC 患者潜在的预后生物标志物。
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