Wang Xiao-Jing, Xiong Ying, Ma Ze-Biao, Xia Jian-Chuan, Li Yan-Fang
Sun Yat-Sen University Cancer Center, State Key Laboratory of Oncology in South China, Collaborative Innovation Center of Cancer Medicine, Guangzhou, 510060, Guangdong, P. R. China.
Department of Gynecologic Oncology, Sun Yat-Sen University Cancer Center, Guangzhou, 510060, Guangdong, P. R. China.
Chin J Cancer. 2016 Jun 16;35(1):54. doi: 10.1186/s40880-016-0114-2.
Protein tyrosine kinase 6 (PTK6) is overexpressed in many epithelial tumors and predicts poor prognosis. However, PTK6 expression status and its role in cervical squamous cell cancer are unknown. This study aimed to investigate the expression level and clinical significance of PTK6 in early-stage cervical squamous cell cancer.
Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) and western blotting analysis were performed to detect PTK6 mRNA and protein expression levels in 10 freshly frozen, early-stage cervical squamous cell cancer specimens and adjacent non-tumorous cervical tissues. The expression of PTK6 was detected using immunohistochemical staining in 150 formalin-fixed, paraffin-embedded, early-stage cervical squamous cell cancer sections and 10 normal cervical tissue sections.
The mRNA and protein levels of PTK6 in cancer tissues were higher than those in adjacent non-tumorous cervical tissues. Immunohistochemical analysis showed that PTK6 was not expressed in normal cervical tissues but was overexpressed in the cytoplasm of cervical squamous cell cancer cells. The level of PTK6 expression was significantly associated with tumor grade (P = 0.020). The 5-year overall survival rate of patients with high PTK6 expression was lower than that of patients with low PTK6 expression (81.3% vs. 96.2%, P = 0.008). Multivariate Cox regression analysis showed that the expression level of PTK6 in cervical squamous cell cancer was an independent prognostic factor for patient survival (hazard ratio = 5.999, 95% confidence interval 1.622-22.191, P < 0.05).
PTK6 is overexpressed in cervical squamous cell cancer. Increased PTK6 expression is associated with reduced 5-year overall survival. PTK6 expression is an independent prognostic predictor for cervical cancer.
蛋白酪氨酸激酶6（PTK6）在许多上皮性肿瘤中过度表达，并预示预后不良。然而，PTK6在宫颈鳞状细胞癌中的表达状态及其作用尚不清楚。本研究旨在探讨PTK6在早期宫颈鳞状细胞癌中的表达水平及临床意义。
采用定量逆转录-聚合酶链反应（qRT-PCR）和蛋白质印迹分析，检测10例新鲜冷冻的早期宫颈鳞状细胞癌标本及相邻非肿瘤性宫颈组织中PTK6 mRNA和蛋白表达水平。采用免疫组织化学染色法检测150例福尔马林固定、石蜡包埋的早期宫颈鳞状细胞癌切片及10例正常宫颈组织切片中PTK6的表达。
癌组织中PTK6的mRNA和蛋白水平高于相邻非肿瘤性宫颈组织。免疫组织化学分析显示，PTK6在正常宫颈组织中不表达，但在宫颈鳞状细胞癌细胞质中过度表达。PTK6表达水平与肿瘤分级显著相关（P = 0.020）。PTK6高表达患者的5年总生存率低于PTK6低表达患者（81.3%对96.2%，P = 0.008）。多因素Cox回归分析显示，宫颈鳞状细胞癌中PTK6的表达水平是患者生存的独立预后因素（风险比=5.999，95%置信区间1.622 - 22.191，P < 0.05）。
PTK6在宫颈鳞状细胞癌中过度表达。PTK6表达增加与5年总生存率降低相关。PTK6表达是宫颈癌的独立预后预测指标。
