Fujita Shigeru
Fujita Dermatological Clinic, Nagaoka City, Niigata, Japan..
Med Mycol J. 2013;54(2):141-6. doi: 10.3314/mmj.54.141.
Slide culture preparations are necessary for accurately identifying dermatophytes. Because the standard slide culture technique is difficult for dermatologists to carry out, there is a need for an economical and easy-to-prepare system without special equipment. We placed a block of agar medium on a square cover glass (24 × 24mm) in the bottom of a case made of polypropylene (Free case, PK-201, HINODEWASHI CO., LTD. Japan). The agar block inoculated with fungi was overlaid with another cover glass on top of it. A plastic petri dish containing 4 cases as described above was sealed with adhesive plastic tape and incubated at 27°C.
载玻片培养制备对于准确鉴定皮肤癣菌是必要的。由于标准的载玻片培养技术对皮肤科医生来说难以实施，因此需要一种无需特殊设备且经济实惠、易于制备的系统。我们将一块琼脂培养基放置在一个由聚丙烯制成的盒子底部的方形盖玻片（24×24毫米）上（免费盒子，PK - 201，日本日之出制纸株式会社）。接种了真菌的琼脂块上面再覆盖另一块盖玻片。一个装有4个上述盒子的塑料培养皿用粘性塑料胶带密封，并在27°C下培养。
