Department of Science Education, Firat University, Elazig 23119, Turkey. fzengin@fi rat.edu.tr
Biol Res. 2013;46(1):79-85. doi: 10.4067/S0716-97602013000100012.
The effects of nickel, cobalt, chromium and zinc on the content of vitamins A, E and C, malondialdehyde (MDA), chlorophyll and carotenoids were investigated in bean seedlings (Phaseolus vulgaris L.) grown in Hoagland solution Control and heavy metal-treated plants were grown for ten days in Hoagland solution. Vitamin A, E, and C content were measured in primary leaves by high performance liquid chromatographic (HPLC). MDA, chlorophyll and carotenoids were measured in leaves by spectrophotometer. In heavy metal treated plants, the levels of MDA, vitamins A, E and C and carotenoids significantly increased, while chlorophyll content decreased in leaves of seedlings. The results indicate that heavy metals caused an oxidative stress in bean plants. The strongest effect on vitamins A, E and C, MDA, chlorophyll and carotenoids was found in plants exposed to nickel, followed by the sequence cobalt > chromium> zinc.
研究了镍、钴、铬和锌对生长在霍格兰溶液中的菜豆（Phaseolus vulgaris L.）幼苗中维生素 A、E 和 C、丙二醛（MDA）、叶绿素和类胡萝卜素含量的影响。对照和重金属处理的植物在霍格兰溶液中生长了十天。通过高效液相色谱法（HPLC）在初生叶中测量维生素 A、E 和 C 的含量。通过分光光度计测量叶片中的 MDA、叶绿素和类胡萝卜素。在重金属处理的植物中，MDA、维生素 A、E 和 C 以及类胡萝卜素的水平显著增加，而幼苗叶片中的叶绿素含量下降。结果表明，重金属导致菜豆植物发生氧化应激。镍处理的植物对维生素 A、E 和 C、MDA、叶绿素和类胡萝卜素的影响最大，其次是钴>铬>锌的顺序。
