Phillipson O T, Sandler M
Brain Res. 1975 Jun 13;90(2):283-96. doi: 10.1016/0006-8993(75)90308-x.
Sympathetic ganglia from 13- to 15-day-old embryonic chicks were cultured for up to 2 days in Leighton tubes. The influence of hydrocortisone and ACTH added to the culture medium on the enzymes monoamine oxidase (MAO) and tyrosine hydroxylase was studied. Hydrocortisone (5 times 10(-5)M) had no effect on tyrosine hydroxylase but increased MAO activity by up to 46 percent over control values under conditions of low or zero nerve growth factor (NGF) concentration. ACTH also increased ganglionic MAO activity, the effect again depending on NGF concentration. This time the maximal response (an increase of 50 percent over controls) was seen at high NGF concentrations. This response was similar to the effect of 1 mM dibutyryl cyclic AMP, and was blocked by 1 times 10-5 M propranolol and 10 muM prostaglandin E(1). ACTH only slightly increased tyrosine hydroxylase activity and this effect was due to a small (18 percent) increase in sympathetic neurone number. Guanosine 5-diphosphate (0.5 mM) was found to increase tyrosine hydroxylase activity by 57 percent and this effect was blocked by the presence of ACTH.
将13至15日龄胚胎鸡的交感神经节在莱顿管中培养长达2天。研究了添加到培养基中的氢化可的松和促肾上腺皮质激素（ACTH）对单胺氧化酶（MAO）和酪氨酸羟化酶的影响。氢化可的松（5×10⁻⁵M）对酪氨酸羟化酶无影响，但在神经生长因子（NGF）浓度低或为零时，MAO活性比对照值增加高达46%。ACTH也增加神经节MAO活性，其作用同样取决于NGF浓度。这一次，在高NGF浓度下观察到最大反应（比对照增加50%）。这种反应类似于1 mM二丁酰环磷酸腺苷的作用，并被1×10⁻⁵M普萘洛尔和10 μM前列腺素E₁阻断。ACTH仅略微增加酪氨酸羟化酶活性，这种作用是由于交感神经元数量小幅增加（18%）。发现鸟苷5-二磷酸（0.5 mM）可使酪氨酸羟化酶活性增加57%，且这种作用被ACTH的存在所阻断。
