针对甲型流感病毒核蛋白的人单克隆抗体免疫意义的体外评估。
In vitro assessment of the immunological significance of a human monoclonal antibody directed to the influenza a virus nucleoprotein.
作者信息
Bodewes Rogier, Geelhoed-Mieras Martina M, Wrammert Jens, Ahmed Rafi, Wilson Patrick C, Fouchier Ron A M, Osterhaus Albert D M E, Rimmelzwaan Guus F
机构信息
Department of Viroscience, Erasmus MC, Rotterdam, the Netherlands.
出版信息
Clin Vaccine Immunol. 2013 Aug;20(8):1333-7. doi: 10.1128/CVI.00339-13. Epub 2013 Jun 12.
Abstract
Influenza A viruses cause annual epidemics and occasionally pandemics. Antibodies directed to the conserved viral nucleoprotein (NP) may play a role in immunity against various influenza A virus subtypes. Here, we assessed the immunological significance of a human monoclonal antibody directed to NP in vitro. This antibody bound to virus-infected cells but did not display virus-neutralizing activity, complement-dependent cell cytotoxicity, or opsonization of viral antigen for improved antigen presentation to CD8(+) T cells by dendritic cells.
摘要
甲型流感病毒引发年度流行，偶尔还会导致大流行。针对保守的病毒核蛋白（NP）的抗体可能在抵抗各种甲型流感病毒亚型的免疫中发挥作用。在此，我们在体外评估了一种针对NP的人单克隆抗体的免疫学意义。该抗体与病毒感染的细胞结合，但不表现出病毒中和活性、补体依赖性细胞毒性或病毒抗原的调理作用，以促进树突状细胞将抗原更好地呈递给CD8(+) T细胞。
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