Graduate Institute of Natural Products, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 80708, Taiwan ; Cancer Center, Kaohsiung Medical University Hospital, Kaohsiung 80756, Taiwan.
Evid Based Complement Alternat Med. 2013;2013:857929. doi: 10.1155/2013/857929. Epub 2013 May 16.
Liriope spicata is a well-known herb in traditional Chinese medicine, and its root has been clinically demonstrated to be effective in the treatment of metabolic and neural disorders. The constituents isolated from Liriope have also recently been shown to possess anticancer activity, although the mechanism of which remains largely unknown. Here, we illustrate the anticancer activity of LPRP-9, one of the active fractions we fractionated from the Liriope platyphylla root part (LPRP) extract. Treatment with LPRP-9 significantly inhibited proliferation of cancer cell lines MCF-7 and Huh-7 and down-regulated the phosphorylation of AKT. LPRP-9 also activates the stress-activated MPAK, JNK, p38 pathways, the p53 cell-cycle checkpoint pathway, and a series of caspase cascades while downregulating expression of antiapoptotic factors Bcl-2, Bcl-XL, and survivin. Such activities strongly suggest a role for LPRP-9 in apoptosis and autophagy. We further purified and identified the compound (-)-Liriopein B from LPRP-9, which is capable of inhibiting AKT phosphorylation at low concentration. The overall result highlights the anticancer property of LPRP-9, suggests its mechanism for inhibition of proliferation and promotion of cell death for cancer cells via regulation of multitarget pathways, and denotes the importance of purifying components of fraction LPRP-9 to aid cancer therapy.
石蒜科植物是一种在传统中药中广为人知的草药，其根部已在临床上证明对代谢和神经紊乱的治疗有效。最近从石蒜中分离出的成分也被证明具有抗癌活性，尽管其机制仍知之甚少。在这里，我们说明了 LPRP-9 的抗癌活性，它是我们从石蒜鳞茎部分（LPRP）提取物中分离的活性部分之一。LPRP-9 的处理显著抑制了 MCF-7 和 Huh-7 癌细胞系的增殖，并下调了 AKT 的磷酸化。LPRP-9 还激活应激激活的 MPAK、JNK、p38 途径、p53 细胞周期检查点途径和一系列半胱天冬酶级联，同时下调抗凋亡因子 Bcl-2、Bcl-XL 和 survivin 的表达。这些活性强烈表明 LPRP-9 在细胞凋亡和自噬中起作用。我们进一步从 LPRP-9 中分离并鉴定了化合物（-）-石蒜苷 B，它能够在低浓度下抑制 AKT 的磷酸化。总的来说，结果突出了 LPRP-9 的抗癌特性，表明其通过调节多靶点途径抑制增殖和促进癌细胞死亡的机制，并强调了纯化 LPRP-9 成分以辅助癌症治疗的重要性。
