Schoepf Karl, Prodinger Wolfgang M, Glawischnig Walter, Hofer Erwin, Revilla-Fernandez Sandra, Hofrichter Johannes, Fritz Johannes, Köfer Josef, Schmoll Friedrich
Austrian Agency for Health and Food Safety (AGES), Institute for Veterinary Disease Control Innsbruck, Technikerstrasse 70, 6020 Innsbruck, Austria.
ISRN Vet Sci. 2012 Oct 22;2012:245138. doi: 10.5402/2012/245138. Print 2012.
A survey of 143 hunter-harvested red deer for tuberculosis was conducted in an Alpine area in Western Austria over two subsequent years. There, single tuberculosis cases caused by Mycobacterium caprae had been detected in cattle and red deer over the preceding decade. The area under investigation covered approximately 500 km(2), divided into five different hunting plots. Lymph nodes of red deer were examined grossly and microscopically for typical tuberculosis-like lesions and additionally by microbiological culturing. Executing a detailed hunting plan, nine M. caprae isolates were obtained. Six out of nine originated from one single hunting plot with the highest estimated prevalence of tuberculosis, that is, 23.1%. All isolates were genotyped by mycobacterial interspersed repetitive unit-variable number of tandem repeat (MIRU-VNTR) typing of 24 standard loci plus VNTR 1982. All nine isolates belonged to a single cluster termed "Lechtal" which had been found in cattle and red deer in the region, demonstrating a remarkable dominance and stability over ten years. This is the first report on a systematic prospective study investigating the prevalence and strain variability of M. caprae infection in red deer in Austria and in the Alpine countries.
在奥地利西部的一个高山地区，连续两年对143头狩猎捕获的马鹿进行了结核病调查。在过去十年中，该地区的牛和马鹿中已检测到由山羊分枝杆菌引起的单例结核病病例。调查区域面积约500平方公里，分为五个不同的狩猎区。对马鹿的淋巴结进行了大体和显微镜检查，以寻找典型的结核样病变，并另外进行了微生物培养。按照详细的狩猎计划，获得了9株山羊分枝杆菌分离株。9株中有6株来自结核病估计患病率最高的一个狩猎区，即23.1%。所有分离株通过对24个标准位点加VNTR 1982进行分枝杆菌散布重复单位-可变数目串联重复序列（MIRU-VNTR）分型进行基因分型。所有9株分离株都属于一个名为“莱希塔尔”的单一聚类，该聚类在该地区的牛和马鹿中都曾发现过，表明其在十年间具有显著的优势和稳定性。这是关于奥地利及阿尔卑斯山国家马鹿中山羊分枝杆菌感染患病率和菌株变异性的系统前瞻性研究的首次报告。
