曲霉属葡糖淀粉酶同工酶对糖原的作用模式

Patterns of action of glucoamylase isozymes from Aspergillus species on glycogen.

作者信息

Gasdorf H J, Atthasampunna P, Dan V, Hensley D E, Smiley K L

出版信息

Carbohydr Res. 1975 Jun;42(1):147-56. doi: 10.1016/s0008-6215(00)84107-5.

Abstract

Glucoamylase isozymes from black Aspergillus species have been freed of all traces of alpha-amylase by chromatography on Bio-Gel P-100, as evidenced by limited hydrolysis of oxidized amylose. Glucoamylase I retains its ability to hydrolyze rabbit-liver glycogen rapidly. By contrast, glucoamylase II hydrolyzes glycogen slowly, and addition of alpha-amylase to glucoamylase II does not enhance its activity toward glycogen. These results indicate that alpha-amylase is not involved in hydrolysis of glycogen by glucoamylase.

摘要

通过在生物凝胶P - 100上进行色谱分析，黑曲霉属物种的葡糖淀粉酶同工酶已去除了所有痕量的α -淀粉酶，这一点可通过氧化直链淀粉的有限水解得到证明。葡糖淀粉酶I保留了快速水解兔肝糖原的能力。相比之下，葡糖淀粉酶II水解糖原的速度较慢，并且向葡糖淀粉酶II中添加α -淀粉酶并不会增强其对糖原的活性。这些结果表明，α -淀粉酶不参与葡糖淀粉酶对糖原的水解过程。