Maeno Y, Takabe F, Inoue H, Iwasa M
Department of Legal Medicine, Nagoya City University Medical School, Japan.
Forensic Sci Int. 1990 Jul;46(3):255-68. doi: 10.1016/0379-0738(90)90311-l.
This paper describes a modified method of simultaneous quantitative determination of histamine and polyamines (putrescine, cadaverine, spermidine and spermine) in incised skin wounds of rats using high-performance liquid chromatography (HPLC) with a strong-cation exchange column, shim-pack ISC-05/S0504P, employing a linear gradient of 0.7-2.5 M sodium chloride. There was scarcely any change in the histamine content of the vital wounds throughout the one week experimental period, although content decreased slightly in 1-h-old wounds. On the other hand, concentrations of putrescine and spermidine increased suddenly 12 h after wounding, and spermine content increased slightly in 4-day-old wounds. There was no post-mortem change in the histamine, spermidine and spermine contents of vital wounds allowed to stand at 25 degrees C up to 24 h after death. Based on these results, this HPLC method may be useful for the determination of wound age during the period of healing.
本文描述了一种改良方法，使用配备强阳离子交换柱（岛津填充ISC - 05/S0504P）的高效液相色谱法（HPLC），采用0.7 - 2.5M氯化钠的线性梯度，同时定量测定大鼠皮肤切口伤口中的组胺和多胺（腐胺、尸胺、亚精胺和精胺）。在整个一周的实验期内，活体伤口中的组胺含量几乎没有变化，尽管在受伤1小时的伤口中含量略有下降。另一方面，腐胺和亚精胺的浓度在受伤12小时后突然增加，精胺含量在4日龄伤口中略有增加。死后将活体伤口在25℃放置长达24小时，其组胺、亚精胺和精胺含量没有死后变化。基于这些结果，这种HPLC方法可能有助于在愈合期间确定伤口年龄。
