The radiometric assay of alkaline phosphatase activity with 125-I-labelled phenolphthalein monophosphate.
作者信息
Aw S E
出版信息
Clin Chim Acta. 1975 May 1;60(3):315-22. doi: 10.1016/0009-8981(75)90073-x.
PMID:237639
Abstract
The coupling of 125-I to phenolphthalein monophosphate is described. Hydrolytic cleavage of the phosphate group by alkaline phosphatases (EC 3.1.-3.1) releases labelled phenolphthalein which is extracted into ethyl acetate at pH 10. This forms the basis of a sensitive assay for the enzyme with the advantages of sensitivity and rapidity from the use of a gamma-emitter. Results with Escherichia coli and calf intestine alkaline phosphatases are presented.
摘要
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