A method for determination of UDP-GlcNAc: GlcNAcβ1-6(GlcNAcβ1-2)Manα1-R [GlcNAc to Man] β1-4N-acetylglucosaminyltransferase VI activity.
作者信息
Taguchi Tomohiko, Taniguchi Naoyuki
机构信息
Department of Health Chemistry, Graduate School of Pharmaceutical Sciences, University of Tokyo, Tokyo, Japan.
出版信息
Methods Mol Biol. 2013;1022:299-305. doi: 10.1007/978-1-62703-465-4_22.
PMID:23765670
Abstract
To characterize and purify glycosyltransferases, it is essential to establish a simple and sensitive assay method. Here, we describe a method for determination of the activity of GnT VI (UDP-GlcNAc: GlcNAcβ1-6(GlcNAcβ1-2)Manα1-R [GlcNAc to Man] β1-4N-acetylglucosaminyltransferase VI) using a fluorescently labeled oligosaccharide.
摘要
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