A method for determination of UDP-GlcNAc: GlcNAcβ1-6(GlcNAcβ1-2)Manα1-R [GlcNAc to Man] β1-4N-acetylglucosaminyltransferase VI activity.
作者信息
Taguchi Tomohiko, Taniguchi Naoyuki
机构信息
Department of Health Chemistry, Graduate School of Pharmaceutical Sciences, University of Tokyo, Tokyo, Japan.
出版信息
Methods Mol Biol. 2013;1022:299-305. doi: 10.1007/978-1-62703-465-4_22.
PMID:23765670
Abstract
To characterize and purify glycosyltransferases, it is essential to establish a simple and sensitive assay method. Here, we describe a method for determination of the activity of GnT VI (UDP-GlcNAc: GlcNAcβ1-6(GlcNAcβ1-2)Manα1-R [GlcNAc to Man] β1-4N-acetylglucosaminyltransferase VI) using a fluorescently labeled oligosaccharide.
摘要
相似文献
1
A method for determination of UDP-GlcNAc: GlcNAcβ1-6(GlcNAcβ1-2)Manα1-R [GlcNAc to Man] β1-4N-acetylglucosaminyltransferase VI activity.
Methods Mol Biol. 2013;1022:299-305. doi: 10.1007/978-1-62703-465-4_22.
2
A method for determination of UDP-GlcNAc: GlcNAc beta 1-6(GlcNAc beta 1-2)Man alpha 1-R [GlcNAc to Man]beta 1-4N-acetylglucosaminyltransferase VI activity using a pyridylaminated tetraantennary oligosaccharide as an acceptor substrate.
Anal Biochem. 1998 Jan 1;255(1):155-7. doi: 10.1006/abio.1997.2465.
3
Purification and characterization of UDP-GlcNAc: GlcNAcbeta 1-6(GlcNAcbeta 1-2)Manalpha 1-R [GlcNAc to Man]-beta 1, 4-N-acetylglucosaminyltransferase VI from hen oviduct.来自母鸡输卵管的UDP-N-乙酰葡糖胺：GlcNAcβ1-6(GlcNAcβ1-2)Manα1-R [GlcNAc至Man]-β1,4-N-乙酰葡糖胺基转移酶VI的纯化与特性鉴定
J Biol Chem. 2000 Oct 20;275(42):32598-602. doi: 10.1074/jbc.M004673200.
4
N-acetylglucosaminyltransferase (GnT) assays using fluorescent oligosaccharide acceptor substrates: GnT-III, IV, V, and IX (GnT-Vb).使用荧光寡糖受体底物的N-乙酰葡糖胺基转移酶（GnT）检测：GnT-III、IV、V和IX（GnT-Vb）。
Methods Mol Biol. 2013;1022:283-98. doi: 10.1007/978-1-62703-465-4_21.
5
Activity of UDP-GlcNAc:GlcNAc beta 1-->6(GlcNAc beta 1-->2) Man alpha 1-->R[GlcNAc to Man] beta 1-->4N-acetylglucosaminyltransferase VI (GnT VI) from the ovaries of Oryzias latipes (Medaka fish).来自青鳉（日本青鳉鱼）卵巢的UDP - 葡萄糖胺：葡萄糖胺β1→6（葡萄糖胺β1→2）甘露糖α1→R[从葡萄糖胺到甘露糖]β1→4N - 乙酰葡糖胺基转移酶VI（GnT VI）的活性
Biochem Biophys Res Commun. 1997 Jan 23;230(3):533-6. doi: 10.1006/bbrc.1996.6013.
6
Molecular cloning and expression of cDNA encoding chicken UDP-N-acetyl-D-glucosamine (GlcNAc): GlcNAcbeta 1-6(GlcNAcbeta 1-2)- manalpha 1-R[GlcNAc to man]beta 1,4N-acetylglucosaminyltransferase VI.编码鸡UDP-N-乙酰-D-葡萄糖胺（GlcNAc）：GlcNAcβ1-6（GlcNAcβ1-2）-甘露糖α1-R[GlcNAc到甘露糖]β1,4-N-乙酰葡糖胺基转移酶VI的cDNA的分子克隆与表达
J Biol Chem. 2000 Nov 17;275(46):36029-34. doi: 10.1074/jbc.M005860200.
7
N-Acetylglucosaminyltransferase IX acts on the GlcNAc beta 1,2-Man alpha 1-Ser/Thr moiety, forming a 2,6-branched structure in brain O-mannosyl glycan.N-乙酰葡糖胺基转移酶IX作用于GlcNAcβ1,2-Manα1-丝氨酸/苏氨酸部分，在脑O-甘露糖聚糖中形成一种2,6-分支结构。
J Biol Chem. 2004 Jan 23;279(4):2337-40. doi: 10.1074/jbc.C300480200. Epub 2003 Nov 14.
8
Molecular cloning and characterization of human GnT-IX, a novel beta1,6-N-acetylglucosaminyltransferase that is specifically expressed in the brain.人类GnT-IX的分子克隆与特性分析，GnT-IX是一种在大脑中特异性表达的新型β1,6-N-乙酰氨基葡萄糖转移酶。
J Biol Chem. 2003 Oct 31;278(44):43102-9. doi: 10.1074/jbc.M308255200. Epub 2003 Aug 26.
9
Cloning and expression of a novel UDP-GlcNAc:alpha-D-mannoside beta1,2-N-acetylglucosaminyltransferase homologous to UDP-GlcNAc:alpha-3-D-mannoside beta1,2-N-acetylglucosaminyltransferase I.一种与UDP-GlcNAc:α-3-D-甘露糖苷β1,2-N-乙酰葡糖胺基转移酶I同源的新型UDP-GlcNAc:α-D-甘露糖苷β1,2-N-乙酰葡糖胺基转移酶的克隆与表达。
Biochem J. 2002 Jan 1;361(Pt 1):153-62. doi: 10.1042/0264-6021:3610153.
10
Synthesis of paucimannose N-glycans by Caenorhabditis elegans requires prior actions of UDP-N-acetyl-D-glucosamine:alpha-3-D-mannoside beta1,2-N-acetylglucosaminyltransferase I, alpha3,6-mannosidase II and a specific membrane-bound beta-N-acetylglucosaminidase.秀丽隐杆线虫合成寡甘露糖型 N-聚糖需要 UDP-N-乙酰-D-葡糖胺：α-3-D-甘露糖苷β1,2-N-乙酰葡糖胺基转移酶 I、α3,6-甘露糖苷酶 II 和一种特定的膜结合β-N-乙酰葡糖胺酶的前期作用。
Biochem J. 2003 May 15;372(Pt 1):53-64. doi: 10.1042/BJ20021931.
Zotero 插件