Li H M, Tang Z X, Zhang H Q, Yan B J, Ren Z L
State Key Laboratory of Plant Genetics and Breeding, Sichuan Agricultural University, Chengdu, Sichuan, China.
Genet Mol Res. 2013 May 21;12(2):1740-51. doi: 10.4238/2013.May.21.5.
Humid rain-fed agriculture is a special environment for wheat (Triticum aestivum) culture that tends to negatively affect wheat yield and quality. To identify quality characters of wheat in a humid environment, we conducted quality analysis and quantitative trait loci (QTL) detection in a recombinant inbred line whose parent had a high level of quality for several years. We found that high-quality wheat had less gluten content and lower protein content. Apparently, wheat quality and associated quantity traits were in a dynamic state of equilibrium. We detected 83 QTL for 10 wheat quality traits in this recombinant inbred line population. Nine QTL were detected in both evaluation years; Q.DT.scau-2A, linked to Xwmc522-2A, was detected at the same genetic location in both years. Other QTL for different traits were detected simultaneously in more than one location. Consequently, there appeared to be pleiotropic genes that control wheat quality. Based on previous studies and our research on QTL analysis of grain protein content, we conclude that there must be one or more genes for grain protein content on chromosome 6B, whose expression was little affected by environment. We constructed a consensus map and projected the QTL on it. It was useful for choosing optimal markers for marker-assisted breeding and map-based cloning.
湿润雨养农业是小麦（普通小麦）种植的一种特殊环境，往往会对小麦产量和品质产生负面影响。为了鉴定湿润环境下小麦的品质性状，我们在一个重组自交系中进行了品质分析和数量性状位点（QTL）检测，该重组自交系的亲本多年来品质水平较高。我们发现，优质小麦的面筋含量较低，蛋白质含量也较低。显然，小麦品质及相关数量性状处于动态平衡状态。我们在这个重组自交系群体中检测到了10个小麦品质性状的83个QTL。在两个评估年份均检测到9个QTL；与Xwmc522-2A连锁的Q.DT.scau-2A在两个年份的相同遗传位置被检测到。不同性状的其他QTL在多个位置同时被检测到。因此，似乎存在控制小麦品质的多效基因。基于先前的研究以及我们对籽粒蛋白质含量QTL分析的研究，我们得出结论，6B染色体上必定存在一个或多个控制籽粒蛋白质含量的基因，其表达受环境影响较小。我们构建了一个整合图谱并将QTL投影到上面。这对于选择用于标记辅助育种和基于图谱克隆的最佳标记很有用。
