Scott D O, Sorensen L R, Lunte C E
Department of Chemistry, University of Kansas, Lawrence, KS 66045.
J Chromatogr. 1990 May 11;506:461-9. doi: 10.1016/s0021-9673(01)91600-0.
In vivo microdialysis sampling coupled to liquid chromatography is a powerful tool for the study of drug metabolism. This technique is illustrated by investigating the pharmacokinetics of acetaminophen in the blood and liver of an anesthetized rat. The pharmacokinetics of the sulfate and glucuronide metabolites as well as the parent acetaminophen can be determined with high precision using microdialysis sampling. Microdialysis samples can be collected at a high rate from several sites without fluid loss with a single animal. Because the animal serves as its own control better data can be obtained. Liquid chromatography provides determination of multiple analytes per sample for metabolic profiling. This technique will provide more accurate and precise pharmacokinetic data while requiring fewer animals.
体内微透析采样与液相色谱联用是研究药物代谢的有力工具。通过研究对乙酰氨基酚在麻醉大鼠血液和肝脏中的药代动力学来说明该技术。使用微透析采样可以高精度地测定硫酸盐和葡萄糖醛酸代谢物以及母体对乙酰氨基酚的药代动力学。微透析样品可以从多个部位以高速率收集，而不会导致单个动物体液流失。由于动物自身作为对照，因此可以获得更好的数据。液相色谱可对每个样品中的多种分析物进行测定，以进行代谢谱分析。该技术将提供更准确和精确的药代动力学数据，同时所需动物数量更少。
