抗生素选择导致铜绿假单胞菌中 nfxC 型表型突变体的意外选择。
Antibiotic selection leads to inadvertent selection of nfxC-type phenotypic mutants in Pseudomonas aeruginosa.
机构信息
BIOMERIT Research Centre, Department of Microbiology, University College Cork, Cork, Ireland. National Laboratory of Plant Engineering and Protein Genetic Engineering, College of Life Science, Peking University, Beijing, China.
出版信息
Environ Microbiol Rep. 2010 Jun;2(3):461-4. doi: 10.1111/j.1758-2229.2010.00175.x.
Spontaneous nfxC-type phenotypic mutants of Pseudomonas aeruginosa are characterized by chloramphenicol resistance and increased expression of the multidrug efflux pump, MexEF-OprN. These mutants frequently arise during the propagation of bacteria in laboratory conditions. This study shows that some of the discrepancies in transcriptomic/phenotypic profiles of two rsmA mutants, PAZH13 (rsmA mutant in strain PAO1) and PAKΔrsmA, are due to the nfxC-type nature of PAZH13. This indicates that awareness of the possible occurrence of these spontaneous nfxC-type phenotypes is necessary. Screening of mexE levels could provide clarity when an nfxC-type phenotype is suspected.
铜绿假单胞菌自发的 nfxC 型表型突变体的特征是对氯霉素的抗性和多药外排泵 MexEF-OprN 的表达增加。这些突变体在实验室条件下细菌繁殖时经常出现。本研究表明，两个 rsmA 突变体（PAO1 菌株中的 rsmA 突变体 PAZH13 和 PAKΔrsmA）的转录组/表型谱中的一些差异是由于 PAZH13 的 nfxC 型性质。这表明，当怀疑出现这种自发的 nfxC 型表型时，有必要意识到可能会发生这种情况。当怀疑出现 nfxC 型表型时，MexE 水平的筛选可以提供明确的结果。
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