Department of Analytical Chemistry, University of Vienna, Währinger Straße 38, 1090 Vienna, Austria.
Food Chem. 2013 Nov 1;141(1):229-35. doi: 10.1016/j.foodchem.2013.02.088. Epub 2013 Mar 7.
The developed duplex real-time PCR method allows the simultaneous detection of traces of potentially allergenic white mustard (Sinapis alba) and celery roots (Apium graveolens var. rapaceum), celery stalks (A. g. var. dulce) and leaf celery (A. g. var. secalinum). The duplex assay does not show any cross-reactivity with 64 different biological species, including various members of the Brassicaceae and Apiaceae family. In raw model sausages spiked with white mustard and celery roots, the LOD was found to be 0.001% white mustard and 0.005% celery. In model sausages brewed at 75-78°C for 15 min the LOD was found to be 0.005% white mustard and 0.005% celery. The duplex real-time PCR assay was applied to check if commercial food products are labelled in compliance with the legal regulations.
开发的双重实时 PCR 方法允许同时检测潜在过敏原白芥菜（Sinapis alba）和芹菜根（Apium graveolens var. rapaceum）、芹菜茎（A. g. var. dulce）和叶芹（A. g. var. secalinum）的痕迹。该双重分析与 64 种不同的生物物种均无交叉反应，包括芸苔科和伞形科的各种成员。在添加了白芥菜和芹菜根的生模型香肠中，检测限为 0.001%白芥菜和 0.005%芹菜。在 75-78°C 下酿造 15 分钟的模型香肠中，检测限为 0.005%白芥菜和 0.005%芹菜。该双重实时 PCR 分析已应用于检查商业食品是否符合法规要求。
