线粒体DNA的不同高变区-1在克隆猪模型中未诱导皮肤同种异体移植排斥反应。

Disparate hypervariable region-1 of mitochondrial DNA did not induce skin allograft rejection in cloned porcine models.

作者信息

Kwak H-H, Park K-M, Nam H-S, Park S-M, Woo H-M

机构信息

Stem Cell Institute, Kangwon National University, ChunCheon, Gangwon-do, Korea.

出版信息

Transplant Proc. 2013 Jun;45(5):1787-91. doi: 10.1016/j.transproceed.2013.01.023.

DOI:10.1016/j.transproceed.2013.01.023

PMID:23769044

Abstract

INTRODUCTION

Alloantigen recognition in skin transplantation is the bane for surgeons. Several studies have mainly focused on the immunogenicity of major histocompatibility (MHC) antigens and H-Y minor histocompatibility antigens. However, the roles of the mitochondrial DNA (mtDNA) encorded miHA have not been identified. Therefore, we sought to address the antigenicity of the hypervariable region 1 (HV-1) of mtDNA in skin transplantation using cloned pig models.

METHODS

Swine leukocyte antigen and HV-1 of mtDNA were analyzed using sequencing methods. Skin transplantation was performed between MHC-matched, mtDNA-mismatched cloned miniature pigs. Full-thickness skin was grafted between cloned pigs without any immunosuppressants. The grafted tissues were observed for 3 months and evaluated histologically.

RESULTS

The cloned pigs shared identical MHC but mtDNA mismatched at 9 positions. Skin grafts between the cloned pigs were accepted and hair growth maintained, whereas MHC-mismatched grafts showed acute rejection within 7 days after transplantation and were replaced by hairless scar tissue.

CONCLUSIONS

HV-1 disparate skin grafts were not recognized as alloantigenic by MHC-matched cloned pigs.

摘要

引言

皮肤移植中的同种异体抗原识别是外科医生面临的难题。多项研究主要聚焦于主要组织相容性（MHC）抗原和H-Y次要组织相容性抗原的免疫原性。然而，线粒体DNA（mtDNA）编码的微小组织相容性抗原（miHA）的作用尚未明确。因此，我们试图利用克隆猪模型探讨皮肤移植中线粒体DNA高变区1（HV-1）的抗原性。