College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China.
J Dairy Sci. 2013 Aug;96(8):4885-90. doi: 10.3168/jds.2013-6550. Epub 2013 Jun 13.
In this study, a method for rapid detection of 5-hydroxymethyl-2-furfural (HMF) was investigated. Monoclonal antibody (anti-HMF) was prepared and evaluated by an indirect competitive ELISA (ic-ELISA) format. The optimized standard curve was y=-0.2097x+1.0432 [where x is the logarithm (base 10) of the values of the HMF concentration and y is the absorbance of ic-ELISA results tested at 490 nm] and the linear detection range was 0.008 to 32.768 mg/L. The percentage of cross-reactivity of HMF with 5 major furfural derivatives was less than 2.92%. Finally, the established ic-ELISA format was used to test HMF in milk, and compared with the result obtained by HPLC, which produced an error of about 0.3%. Based on the data in this experiment, we concluded that the established ic-ELISA format was reliable with a high specificity.
本研究旨在探索一种快速检测 5-羟甲基糠醛（HMF）的方法。通过间接竞争酶联免疫吸附试验（ic-ELISA）的形式，制备并评估了单克隆抗体（抗-HMF）。优化后的标准曲线为 y=-0.2097x+1.0432 [其中 x 为 HMF 浓度的以 10 为底的对数，y 为在 490nm 下检测到的 ic-ELISA 结果的吸光度]，线性检测范围为 0.008 至 32.768mg/L。HMF 与 5 种主要糠醛衍生物的交叉反应率均小于 2.92%。最后，将建立的 ic-ELISA 格式用于检测牛奶中的 HMF，并与 HPLC 结果进行比较，误差约为 0.3%。基于本实验中的数据，我们得出结论，所建立的 ic-ELISA 格式具有较高的特异性，结果可靠。
