Graduate School of Agricultural Science, Kobe University, Kobe 657-8501, Japan.
Comp Biochem Physiol B Biochem Mol Biol. 2013 Sep;166(1):1-6. doi: 10.1016/j.cbpb.2013.06.003. Epub 2013 Jun 14.
The aim of this study was to analyze the expression patterns of key genes involved in lipid metabolism in response to feeding in chicks. A total of 18 thirteen day-old male chicks were fasted for 12h. The mRNA levels of the genes in the liver and white adipose tissue were analyzed after 0, 2, and 4h of refeeding. The mRNA levels of sterol regulatory element-binding protein (SREBP) 1, liver X receptor α, peroxisome proliferator-activated receptor (PPAR) γ, acetyl-CoA carboxylase α and fatty acid synthase were significantly increased after 2h of refeeding. In contrast, the mRNA levels of PPARα and carnitine palmitoyltransferase 1a were significantly decreased after 2h of refeeding. The mRNA level of acyl-CoA oxidase was significantly decreased after 4h of refeeding. The mRNA levels of cholesterol metabolism-related genes such as SREBP2 and 3-hydroxy-3-methylglutaryl-CoA reductase were significantly increased after 2h of refeeding. In the white adipose tissue, the mRNA level of PPARγ was significantly increased after 2h of refeeding, whereas the mRNA level of adipose triglyceride lipase was significantly decreased after 4h of refeeding. These results demonstrated that expression of lipid metabolism-related genes is regulated by short-term refeeding in chicks.
本研究旨在分析在小鸡进食过程中涉及脂质代谢的关键基因的表达模式。共选择 18 只 13 日龄雄性小鸡进行 12h 的禁食，再分别于复食后 0、2、4h 检测肝脏和白色脂肪组织中基因的 mRNA 水平。复食 2h 后，固醇调节元件结合蛋白 1（SREBP）1、肝 X 受体α、过氧化物酶体增殖物激活受体（PPAR）γ、乙酰辅酶 A 羧化酶α和脂肪酸合酶的 mRNA 水平显著增加。相反，复食 2h 后，PPARα和肉碱棕榈酰基转移酶 1a 的 mRNA 水平显著降低。复食 4h 后，酰基辅酶 A 氧化酶的 mRNA 水平显著降低。复食 2h 后，胆固醇代谢相关基因 SREBP2 和 3-羟-3-甲基戊二酰辅酶 A 还原酶的 mRNA 水平显著增加。在白色脂肪组织中，复食 2h 后 PPARγ 的 mRNA 水平显著增加，而 4h 后脂肪甘油三酯脂酶的 mRNA 水平显著降低。这些结果表明，脂质代谢相关基因的表达受小鸡短期进食的调控。
