CSIRO Plant Industry, GPO Box 1600, Canberra, ACT, Australia.
FEBS Lett. 2013 Aug 2;587(15):2371-6. doi: 10.1016/j.febslet.2013.06.003. Epub 2013 Jun 13.
Demonstration of the function of the Arabidopsis thaliana acyl-CoA:diacylglycerol acyltransferase 2 (AtDGAT2) has remained elusive despite biochemical testing of genetic mutants and overexpression lines. We show that transiently expressed AtDGAT2 in the Nicotiana benthamiana leaf resulted in an increase in triacylglycerol twice as great as the increase observed following parallel expression of AtDGAT1. AtDGAT2 showed higher conversion from labeled diacylglycerol to triacylglycerol compared to AtDGAT1, and was acyl-CoA dependent. In addition, AtDGAT2 had different acyl-CoA substrate preference than AtDGAT1. These results allow us to conclude that AtDAGT2 is a functional acyl-CoA:diacylglycerol acyltransferase enzyme that can catalyse substantial increase in TAG synthesis.
尽管对遗传突变体和过表达系进行了生化测试，但拟南芥酰基辅酶 A：二酰基甘油酰基转移酶 2（AtDGAT2）的功能仍未得到证实。我们表明，在烟草 Nicotiana benthamiana 叶中瞬时表达 AtDGAT2 导致三酰基甘油增加的幅度是平行表达 AtDGAT1 时观察到的增加幅度的两倍。与 AtDGAT1 相比，AtDGAT2 从标记的二酰基甘油转化为三酰基甘油的转化率更高，并且依赖酰基辅酶 A。此外，AtDGAT2 对酰基辅酶 A 的底物偏好与 AtDGAT1 不同。这些结果使我们得出结论，AtDAGT2 是一种具有功能的酰基辅酶 A：二酰基甘油酰基转移酶，能够催化 TAG 合成的大量增加。
