Department of Clinical Medicine, Faculty of Medicine, Universidade Federal de Pernambuco (UFPE), Brazil.
Hum Immunol. 2013 Sep;74(9):1153-6. doi: 10.1016/j.humimm.2013.05.015. Epub 2013 Jun 12.
To investigate the association of the IL-6 gene promoter polymorphism (-174 G/C) with SLE susceptibility and disease features in Brazilian patients, a case-control study of 80 lupus cases and 60 volunteer healthy women was performed. Genotyping was carried out by polymerase chain reaction and PCR product was digested by HSP92II restriction enzyme, being after visualized in polyacrylamide gel. There were significant differences in the distribution of the IL-6 gene C/C polymorphism between the SLE and control groups (χ(2) = 8.668; P = 0.0032). Individual carriers of the variant allele G had a 1.98 (95% CI: 1.0844-3.6353)-fold increased risk for SLE. Besides, association was observed in frequency of C allele (P = 0.0247; OR = 0.5036) between SLE patients and control groups. This study presents preliminary evidence for association between IL-6 polymorphism and SLE susceptibility in a Northeast population from Brazil.
为了探讨白细胞介素 6（IL-6）基因启动子多态性（-174 G/C）与巴西患者系统性红斑狼疮（SLE）易感性和疾病特征的关系，进行了一项病例对照研究，共纳入 80 例狼疮患者和 60 名志愿者健康女性。采用聚合酶链反应（PCR）进行基因分型，PCR 产物用 HSP92II 限制酶消化，然后在聚丙烯酰胺凝胶中可视化。SLE 组和对照组之间 IL-6 基因 C/C 多态性的分布存在显著差异（χ(2) = 8.668；P = 0.0032）。变异等位基因 G 的个体携带者患 SLE 的风险增加了 1.98 倍（95%CI：1.0844-3.6353）。此外，SLE 患者和对照组之间 C 等位基因的频率也存在关联（P = 0.0247；OR = 0.5036）。本研究初步证明了 IL-6 多态性与巴西东北部人群 SLE 易感性之间存在关联。
