Department of Anatomy, Shandong University School of Medicine, Jinan 250012, China.
Neurochem Int. 2013 Sep;63(3):146-53. doi: 10.1016/j.neuint.2013.05.011. Epub 2013 Jun 13.
Schwann cells (SCs) are the myelin forming cells in the peripheral nervous system, they play a key role in the pathology of various polyneuropathies and provide trophic support to axons via expression of various neurotrophic factors, such as nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF) and glial cell line-derived neurotrophic factor (GDNF). Ethanol (EtOH) adversely affected both SCs proliferation and myelin formation in culture. Resveratrol (Res) has been shown to regulate many cellular processes and to display multiple protective and therapeutic effects. Whether Res has protective effects on SCs with EtOH-induced toxicity is still unclear. The protective efficacy of Res on EtOH-treated SCs in vitro was investigated in the present study. Res improved cell viability of the EtOH-treated SCs. Hoechst 33342 staining and terminal deoxynucleotidyl transferase (TdT)-mediated deoxyuridine triphosphate nick-end labeling analysis showed that the EtOH-induced apoptosis was inhibited by Res. The effects of Res were blocked by the 5'-adenosine monophosphate-activated protein kinase inhibitor Compound C and the silencing information regulator T1 inhibitor nicotinamide. Res could increase the mRNA and protein levels of BDNF and GDNF in the EtOH-treated SCs. However, the EtOH-induced increase of NGF in the SCs is inhibited by Res. The data from the present study indicate that Res protects SCs from EtOH-induced cell death and regulates the expression of neurotrophicfactors. Res and its derivative may be effective for the treatment of neuropathic diseases induced by EtOH.
许旺细胞（SCs）是周围神经系统中的髓鞘形成细胞，它们在各种多发性神经病的病理学中起着关键作用，并通过表达各种神经营养因子（如神经生长因子（NGF）、脑源性神经营养因子（BDNF）和胶质细胞系衍生的神经营养因子（GDNF））为轴突提供营养支持。乙醇（EtOH）在培养物中对 SCs 的增殖和髓鞘形成均有不利影响。白藜芦醇（Res）已被证明可调节多种细胞过程，并具有多种保护和治疗作用。Res 是否对 EtOH 诱导的毒性的 SCs 具有保护作用尚不清楚。本研究旨在探讨 Res 对 EtOH 处理的 SCs 的体外保护作用。Res 提高了 EtOH 处理的 SCs 的细胞活力。Hoechst 33342 染色和末端脱氧核苷酸转移酶（TdT）介导的脱氧尿苷三磷酸缺口末端标记分析表明，Res 抑制了 EtOH 诱导的细胞凋亡。5'-腺苷单磷酸激活蛋白激酶抑制剂 Compound C 和沉默信息调节因子 T1 抑制剂烟酰胺阻断了 Res 的作用。Res 可增加 EtOH 处理的 SCs 中 BDNF 和 GDNF 的 mRNA 和蛋白水平。然而，Res 抑制了 EtOH 诱导的 SCs 中 NGF 的增加。本研究的数据表明，Res 可保护 SCs 免受 EtOH 诱导的细胞死亡，并调节神经营养因子的表达。Res 及其衍生物可能对治疗由 EtOH 引起的神经病变有效。
