Istanbul University, Istanbul Faculty of Medicine, Physiology Department, Istanbul, Turkey.
Cell Immunol. 2013 Apr;282(2):93-9. doi: 10.1016/j.cellimm.2013.04.012. Epub 2013 May 4.
The physiological mechanisms of decreased NK activity of β-Thalassemia major (BTM) patients are unknown. To assess in vitro effects of mononuclear cells and their cytokine secretion on NK activity, we compared activator receptor levels and cytotoxic activity of purified NK cells and NK cells in mononuclear cells (MNC) pools. We collected cell supernatant from unincubated and incubated MNC with K562 cells and measured their secreted cytokines levels. CD16 was lower on the surface of NK cells in MNC pools from BTM patients compared to healthy volunteers. This inhibition does not appear when NK cells were purified. NKp30 levels in NK cells decreased both as purified cells and as part of a pool of MNC in BTM patients. After incubation of MNC pools with K562 target cells, we found that supernatant levels of IL10, TGFβ1 and IL15 cytokines were also significantly higher in BTM patients compared to healthy volunteers.
β-地中海贫血（BTM）患者 NK 活性降低的生理机制尚不清楚。为了评估单核细胞及其细胞因子分泌对 NK 活性的体外影响，我们比较了纯化 NK 细胞和单核细胞（MNC）池中的 NK 细胞的激活受体水平和细胞毒性活性。我们从未孵育和孵育的 MNC 与 K562 细胞中收集细胞上清液，并测量其分泌的细胞因子水平。与健康志愿者相比，BTM 患者 MNC 池中的 NK 细胞表面的 CD16 水平较低。当纯化 NK 细胞时，这种抑制作用不会出现。NKp30 水平在作为纯化细胞和作为 MNC 池的一部分的 BTM 患者的 NK 细胞中均降低。孵育 MNC 池与 K562 靶细胞后，我们发现上清液中白细胞介素 10（IL10）、转化生长因子 β1（TGFβ1）和白细胞介素 15（IL15）细胞因子的水平在 BTM 患者中也明显高于健康志愿者。
