• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

不同程度 NOX2 调节的受试者血小板与尿 8-异前列腺素 F2α 水平的关系。

Relationship between platelet and urinary 8-Iso-PGF2α levels in subjects with different degrees of NOX2 regulation.

机构信息

Clinica Medica, University of Rome "La Sapienza", Rome, Italy.

出版信息

J Am Heart Assoc. 2013 Jun 14;2(3):e000198. doi: 10.1161/JAHA.113.000198.

DOI:10.1161/JAHA.113.000198
PMID:23770972
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3698784/
Abstract

BACKGROUND

Urinary 8-iso-PGF2α, a marker of oxidative stress, is influenced by the activation of NOX2. It is unclear if platelets 8-iso-PGF2α contribute to urinary 8-iso-PGF2α.

METHODS AND RESULTS

In a cross-sectional study, platelet, urinary, and serum 8-iso-PGF2α were determined in subjects with downregulation (X-linked chronic granulomatous disease [X-CGD], n=25) and upregulation (type II diabetic patients [T2D], n=121) of NOX2 and 153 controls matched for sex and age. In diabetic patients (n=18), the above variables were repeated before and after 7 days treatment with 100 mg/day aspirin or 100 mg/day aspirin plus 40 mg/day atorvastatin. In vitro study was performed to see the contribution of blood cells to serum 8-iso-PGF2α. Compared with controls, X-CGD patients had lower platelet, serum, and urinary 8-iso-PGF2α values; conversely, diabetic patients had higher values of 8-iso-PGF2α compared with controls. Urinary 8-iso-PGF2α significantly correlated with both platelet and serum 8-iso-PGF2α in the 2 cohorts. A parallel increase of platelet, serum, and urinary 8-iso-PGF2α by aspirin and a parallel decrease by aspirin plus atorvastatin were detected in the interventional study. In vitro study demonstrated that platelets contribute to 37% of serum 8-iso-PGF2α and that only 13% of it is of extravascular origin.

CONCLUSIONS

The study suggests that NOX2 contributes to the formation of 8-iso-PGF2α in both platelets and urine. The direct correlation between platelet and urinary 8-iso-PGF2α suggests that, at least partly, urinary 8-iso-PGF2α reflects platelet 8-iso-PGF2α production. Analysis of serum 8-iso-PGF2α may represent a novel tool to investigate the production of 8-iso-PGF2α by blood cells including platelets.

CLINICAL TRIAL REGISTRATION

URL: ClinicalTrials.gov. Unique Identifier: NCT01250340.

摘要

背景

尿液 8-异前列腺素 F2α（8-iso-PGF2α）是氧化应激的标志物，其水平受 NOX2 激活的影响。目前尚不清楚血小板 8-iso-PGF2α 是否会导致尿液 8-iso-PGF2α 升高。

方法和结果

在一项横断面研究中，检测了下调（X 连锁慢性肉芽肿病[X-CGD]，n=25）和上调（2 型糖尿病患者[T2D]，n=121）NOX2 以及 153 名性别和年龄匹配的对照组的血小板、尿液和血清 8-iso-PGF2α。在 18 例糖尿病患者中，在服用 100 mg/天阿司匹林或 100 mg/天阿司匹林加 40 mg/天阿托伐他汀治疗前和治疗后 7 天重复了上述变量。进行了体外研究以观察血细胞对血清 8-iso-PGF2α 的贡献。与对照组相比，X-CGD 患者的血小板、血清和尿液 8-iso-PGF2α 水平较低；相反，糖尿病患者的 8-iso-PGF2α 水平高于对照组。在这两个队列中，尿液 8-iso-PGF2α 与血小板和血清 8-iso-PGF2α 均呈显著相关。在干预研究中，我们发现阿司匹林可使血小板、血清和尿液 8-iso-PGF2α 呈平行增加，而阿司匹林加阿托伐他汀可使 8-iso-PGF2α 呈平行降低。体外研究表明，血小板占血清 8-iso-PGF2α 的 37%，而仅有 13%来自血管外。

结论

该研究表明，NOX2 参与了血小板和尿液中 8-iso-PGF2α 的形成。血小板和尿液 8-iso-PGF2α 之间的直接相关性表明，至少部分情况下，尿液 8-iso-PGF2α 反映了血小板 8-iso-PGF2α 的产生。分析血清 8-iso-PGF2α 可能代表一种新的工具，用于研究包括血小板在内的血细胞产生 8-iso-PGF2α。

临床试验注册

网址：ClinicalTrials.gov。唯一标识符：NCT01250340。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/137d/3698784/b6e6cd06e0c8/jah3-2-e000198-g5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/137d/3698784/5b55195a8fbb/jah3-2-e000198-g1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/137d/3698784/84c4e00c4b2d/jah3-2-e000198-g2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/137d/3698784/e6755ecd9fc4/jah3-2-e000198-g3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/137d/3698784/63252ba65432/jah3-2-e000198-g4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/137d/3698784/b6e6cd06e0c8/jah3-2-e000198-g5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/137d/3698784/5b55195a8fbb/jah3-2-e000198-g1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/137d/3698784/84c4e00c4b2d/jah3-2-e000198-g2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/137d/3698784/e6755ecd9fc4/jah3-2-e000198-g3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/137d/3698784/63252ba65432/jah3-2-e000198-g4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/137d/3698784/b6e6cd06e0c8/jah3-2-e000198-g5.jpg

相似文献

1
Relationship between platelet and urinary 8-Iso-PGF2α levels in subjects with different degrees of NOX2 regulation.不同程度 NOX2 调节的受试者血小板与尿 8-异前列腺素 F2α 水平的关系。
J Am Heart Assoc. 2013 Jun 14;2(3):e000198. doi: 10.1161/JAHA.113.000198.
2
Different degrees of NADPH oxidase 2 regulation and in vivo platelet activation: lesson from chronic granulomatous disease.不同程度的NADPH氧化酶2调节与体内血小板活化：来自慢性肉芽肿病的教训
J Am Heart Assoc. 2014 Jun 27;3(3):e000920. doi: 10.1161/JAHA.114.000920.
3
NOX2-generated oxidative stress is associated with severity of ultrasound liver steatosis in patients with non-alcoholic fatty liver disease.NOX2产生的氧化应激与非酒精性脂肪性肝病患者肝脏超声脂肪变性的严重程度相关。
BMC Gastroenterol. 2014 Apr 23;14:81. doi: 10.1186/1471-230X-14-81.
4
Short-term in vivo modifications of platelet NADPH oxidase 2 (NOX2) and prostaglandin F in HIV-1 patients on abacavir-based therapies.接受基于阿巴卡韦疗法的HIV-1患者血小板NADPH氧化酶2（NOX2）和前列腺素F的短期体内变化
HIV Med. 2016 Nov;17(10):774-777. doi: 10.1111/hiv.12383.
5
Serum NOX2 and urinary isoprostanes predict vascular events in patients with atrial fibrillation.血清NOX2和尿异前列腺素可预测房颤患者的血管事件。
Thromb Haemost. 2015 Mar;113(3):617-24. doi: 10.1160/TH14-07-0571. Epub 2014 Nov 13.
6
Endothelial dysfunction and oxidative stress in children with sleep disordered breathing: role of NADPH oxidase.睡眠呼吸障碍儿童的内皮功能障碍与氧化应激：NADPH氧化酶的作用
Atherosclerosis. 2015 May;240(1):222-7. doi: 10.1016/j.atherosclerosis.2015.03.024. Epub 2015 Mar 18.
7
In vivo formation of 8-iso-prostaglandin f2alpha and platelet activation in diabetes mellitus: effects of improved metabolic control and vitamin E supplementation.糖尿病患者体内8-异前列腺素F2α的形成及血小板活化：代谢控制改善和补充维生素E的影响
Circulation. 1999 Jan 19;99(2):224-9. doi: 10.1161/01.cir.99.2.224.
8
Dark chocolate inhibits platelet isoprostanes via NOX2 down-regulation in smokers.黑巧克力通过下调烟民体内的 NOX2 抑制血小板异前列烷生成。
J Thromb Haemost. 2012 Jan;10(1):125-32. doi: 10.1111/j.1538-7836.2011.04558.x.
9
Thromboxane-dependent CD40 ligand release in type 2 diabetes mellitus.2型糖尿病中血栓素依赖性CD40配体释放
J Am Coll Cardiol. 2006 Jan 17;47(2):391-7. doi: 10.1016/j.jacc.2005.03.079. Epub 2005 Dec 20.
10
Lipopolysaccharide as trigger of platelet aggregation via eicosanoid over-production.脂多糖通过类花生酸过度产生触发血小板聚集。
Thromb Haemost. 2017 Jul 26;117(8):1558-1570. doi: 10.1160/TH16-11-0857. Epub 2017 May 11.

引用本文的文献

1
The Role of Antioxidants Supplementation in Clinical Practice: Focus on Cardiovascular Risk Factors.抗氧化剂补充在临床实践中的作用：关注心血管危险因素。
Antioxidants (Basel). 2021 Jan 20;10(2):146. doi: 10.3390/antiox10020146.
2
Oxidative stress: New insights on the association of non-alcoholic fatty liver disease and atherosclerosis.氧化应激：非酒精性脂肪性肝病与动脉粥样硬化关联的新见解
World J Hepatol. 2015 Jun 8;7(10):1325-36. doi: 10.4254/wjh.v7.i10.1325.
3
Moderate physical training attenuates perinatal low-protein-induced spleen lymphocyte apoptosis in endotoxemic adult offspring rats.

本文引用的文献

1
Platelet isoprostane overproduction in diabetic patients treated with aspirin.糖尿病患者应用阿司匹林治疗后血小板异前列烷生成增加。
Diabetes. 2012 Jun;61(6):1626-32. doi: 10.2337/db11-1243. Epub 2012 Mar 16.
2
Diagnosis and classification of diabetes mellitus.糖尿病的诊断与分类
Diabetes Care. 2011 Jan;34 Suppl 1(Suppl 1):S62-9. doi: 10.2337/dc11-S062.
3
Inherited human gp91phox deficiency is associated with impaired isoprostane formation and platelet dysfunction.遗传性人 gp91phox 缺陷与异前列腺素形成受损和血小板功能障碍有关。
适度体育锻炼可减轻围产期低蛋白饮食诱导的内毒素血症成年子代大鼠脾脏淋巴细胞凋亡。
Eur J Nutr. 2016 Apr;55(3):1113-22. doi: 10.1007/s00394-015-0925-y. Epub 2015 May 19.
4
Low dose of acetylsalicylic acid and oxidative stress-mediated endothelial dysfunction in diabetes: a short-term evaluation.低剂量乙酰水杨酸与糖尿病中氧化应激介导的内皮功能障碍：短期评估
Acta Diabetol. 2015 Apr;52(2):249-56. doi: 10.1007/s00592-014-0629-4. Epub 2014 Aug 6.
5
Different degrees of NADPH oxidase 2 regulation and in vivo platelet activation: lesson from chronic granulomatous disease.不同程度的NADPH氧化酶2调节与体内血小板活化：来自慢性肉芽肿病的教训
J Am Heart Assoc. 2014 Jun 27;3(3):e000920. doi: 10.1161/JAHA.114.000920.
Arterioscler Thromb Vasc Biol. 2011 Feb;31(2):423-34. doi: 10.1161/ATVBAHA.110.217885. Epub 2010 Nov 11.
4
Atorvastatin inhibits oxidative stress via adiponectin-mediated NADPH oxidase down-regulation in hypercholesterolemic patients.阿托伐他汀通过脂联素介导的 NADPH 氧化酶下调抑制高胆固醇血症患者的氧化应激。
Atherosclerosis. 2010 Nov;213(1):225-34. doi: 10.1016/j.atherosclerosis.2010.08.056. Epub 2010 Aug 19.
5
Atorvastatin inhibits gp91phox circulating levels in patients with hypercholesterolemia.阿托伐他汀抑制高胆固醇血症患者循环 gp91phox 水平。
Arterioscler Thromb Vasc Biol. 2010 Feb;30(2):360-7. doi: 10.1161/ATVBAHA.109.198622. Epub 2009 Dec 3.
6
Hereditary deficiency of gp91(phox) is associated with enhanced arterial dilatation: results of a multicenter study.gp91（吞噬细胞氧化酶）遗传性缺陷与动脉扩张增强有关：一项多中心研究结果
Circulation. 2009 Oct 20;120(16):1616-22. doi: 10.1161/CIRCULATIONAHA.109.877191. Epub 2009 Oct 5.
7
Clinical features, long-term follow-up and outcome of a large cohort of patients with Chronic Granulomatous Disease: an Italian multicenter study.一大群慢性肉芽肿病患者的临床特征、长期随访及转归：一项意大利多中心研究
Clin Immunol. 2008 Feb;126(2):155-64. doi: 10.1016/j.clim.2007.09.008. Epub 2007 Nov 26.
8
Isoprostane measurement in plasma and urine by liquid chromatography-mass spectrometry with one-step sample preparation.采用一步样品制备的液相色谱-质谱法测定血浆和尿液中的异前列腺素。
Clin Chem. 2007 Feb;53(2):251-8. doi: 10.1373/clinchem.2006.074989. Epub 2007 Jan 2.
9
Low-dose aspirin for the prevention of atherothrombosis.小剂量阿司匹林用于预防动脉粥样硬化血栓形成。
N Engl J Med. 2005 Dec 1;353(22):2373-83. doi: 10.1056/NEJMra052717.
10
Oxidative stress and cardiovascular injury: Part II: animal and human studies.氧化应激与心血管损伤：第二部分：动物与人体研究
Circulation. 2003 Oct 28;108(17):2034-40. doi: 10.1161/01.CIR.0000093661.90582.c4.