Department of Animal Sciences, The Ohio State University, Columbus, OH 43210, United States.
Bioresour Technol. 2013 Aug;142:625-32. doi: 10.1016/j.biortech.2013.05.090. Epub 2013 May 30.
A temperature-phased anaerobic digestion system with the thermophilic digester acidified by acidogenesis products (referred to as AT-TPAD) was evaluated to treat high-strength dairy cattle manure at a 15-day retention time. Three temperatures (50, 55, and 60°C) were tested on the thermophilic digester, and 50°C was found to be the optimal temperature for overall performance of the AT-TPAD system, achieving 31% VS removal and 0.22 L methane/g VS fed. The mesophilic digester contributed significantly more to the overall system performance than the thermophilic digester. The thermophilic and the mesophilic digesters had different microbial communities under all conditions, and both microbial communities exhibited dynamic changes in response to different conditions. Certain microbial groups were found significantly correlated with the system performance. Methanosarcina was the most important methanogen genus of the AT-TPAD system and its population abundance was inversely correlated with high concentrations of volatile fatty acids (VFA).
采用温度分相厌氧消化系统，利用产酸发酵产物酸化高温消化器（简称 AT-TPAD），在 15 天停留时间内处理高强度奶牛粪便。在高温消化器中测试了三种温度（50、55 和 60°C），发现 50°C 是 AT-TPAD 系统整体性能的最佳温度，实现了 31%VS 去除率和 0.22 L 甲烷/gVS 进料。中温消化器对整个系统性能的贡献明显大于高温消化器。在所有条件下，高温消化器和中温消化器中的微生物群落均不同，并且两个微生物群落都对不同条件表现出动态变化。发现某些微生物群与系统性能显著相关。产甲烷菌是 AT-TPAD 系统中最重要的产甲烷菌属，其种群丰度与高浓度挥发性脂肪酸（VFA）呈负相关。
