Department of Animal Sciences, The Ohio State University, Columbus, OH 43210, USA.
Bioresour Technol. 2013 Sep;143:431-8. doi: 10.1016/j.biortech.2013.06.013. Epub 2013 Jun 13.
Two temperature-phased anaerobic digestion (TPAD) systems, with the thermophilic digesters acidified by acidogenesis products (AT-TPAD) or operated at neutral pH and balanced hydrolysis/acidogenesis and methanogenesis (NT-TPAD), were evaluated to treat high-strength dairy cattle manure. Despite similar methane productions (about 0.22 L/g VS fed), the NT-TPAD system removed significantly more VS (36%) than the AT-TPAD system (31%) and needed no pH adjustments. The thermophilic digester of the NT-TPAD system dominated the system performance and performed significantly better than that of the AT-TPAD system. The opposite held true for the mesophilic digesters. Differences of the thermophilic digesters between two TPAD systems affected the microbial communities of both local and downstream digesters. Each digester harbored distinctive microbial populations, some of which were significantly correlated with system performance. Methanosarcina was the most important methanogenic genus in both TPAD systems, while Methanosaeta only in the NT-TPAD system. Their populations were inversely related to VFA concentrations.
两种温度分相厌氧消化（TPAD）系统，分别采用产酸相酸化的高温消化器（AT-TPAD）或中性 pH 值且水解/产酸和产甲烷相平衡的高温消化器（NT-TPAD），被用来处理高浓度奶牛粪便。尽管甲烷产量相似（约 0.22 L/g VS 进料），但 NT-TPAD 系统比 AT-TPAD 系统去除更多的挥发性固体（36% VS 31%），并且不需要进行 pH 调整。NT-TPAD 系统的高温消化器主导了系统性能，并且明显优于 AT-TPAD 系统。这种情况在中温消化器中正好相反。两种 TPAD 系统的高温消化器的差异影响了局部和下游消化器的微生物群落。每个消化器都有独特的微生物种群，其中一些与系统性能显著相关。甲烷八叠球菌是两种 TPAD 系统中最重要的产甲烷菌属，而甲烷丝菌仅存在于 NT-TPAD 系统中。它们的种群与 VFA 浓度呈反比关系。
