Ling Jia-Yan, Shen Lin, Liu Qing, Xue Sha, Ma Wei, Wu Hui, Li Zi-Xi, Zhu Rui
Department of Integrated Chinese and Western Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China.
Department of Chinese Medicine, the First Hospital of Wuhan, Wuhan, 430022, China.
J Huazhong Univ Sci Technolog Med Sci. 2013 Jun;33(3):438-442. doi: 10.1007/s11596-013-1138-3. Epub 2013 Jun 17.
Glycoprotein (GP) Ibα ectodomain shedding has important implications for thrombosis and hemostasis. A disintegrin and metalloproteinase 17 (ADAM17) was identified to play an essential role in agonist induced GPIbα shedding. The relationship of GPIbα shedding and ADAM17 in the acute stage of atherosclerotic ischemic stroke (AIS) patients has not been thoroughly studied. A total of 306 patients and 230 controls matched for age, sex, race, history of hypertension and diabetes mellitus were enrolled in the study. GPIbα, ADAM17, glycocalicin were detected by flow cytometry, Western blotting, and enzyme-linked immunosorbent assay (ELISA) respectively. Compared with the control group, the expression of GPIbα in patients with acute ischemic stroke was significantly lower (P=0.000, P<0.01). Plasma glycocalicin and ADAM17 in AIS group were higher than those in control group (P=0.699, P=0.000). Pearson's analysis showed glycocalicin bore no correlation with GPIbα in AIS patients (r=0.095, P>0.05). GPIbα and National Institute of Health Stroke Scale (NIHSS) had negative correlation (r=-0.514, P<0.01). Our findings indicate that ADAM17 may be a risk factor for ischemic stroke in Chinese and the expression of GPIbα can serve as a measure for stroke severity.
糖蛋白（GP）Ibα胞外域脱落对血栓形成和止血具有重要意义。已确定解整合素和金属蛋白酶17（ADAM17）在激动剂诱导的GPIbα脱落中起关键作用。动脉粥样硬化性缺血性卒中（AIS）患者急性期GPIbα脱落与ADAM17的关系尚未得到充分研究。本研究共纳入306例患者及230名年龄、性别、种族、高血压和糖尿病病史相匹配的对照者。分别采用流式细胞术、蛋白质免疫印迹法和酶联免疫吸附测定（ELISA）检测GPIbα、ADAM17和糖钙蛋白。与对照组相比，急性缺血性卒中患者GPIbα表达显著降低（P = 0.000，P<0.01）。AIS组血浆糖钙蛋白和ADAM17高于对照组（P = 0.699，P = 0.000）。Pearson分析显示，AIS患者中糖钙蛋白与GPIbα无相关性（r = 0.095，P>0.05）。GPIbα与美国国立卫生研究院卒中量表（NIHSS）呈负相关（r = -0.514，P<0.01）。我们的研究结果表明，ADAM17可能是中国人群缺血性卒中的一个危险因素，GPIbα的表达可作为卒中严重程度的一个衡量指标。
