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尼莫地平可挽救N-甲基-N-亚硝基脲诱导的大鼠视网膜变性。

Nimodipine rescues N-methyl-N-nitrosourea-induced retinal degeneration in rats.

作者信息

Wang Dan, Li Yan, Wang Zhuo, Sun Gui-Yuan, Zhang Qiu-Hua

机构信息

Department of Histology and Embryology, College of Basic Medical Science, China Medical University, P. R. China ; Department of Pharmacology, HE's University, P. R. China.

出版信息

Pharmacogn Mag. 2013 Apr;9(34):149-54. doi: 10.4103/0973-1296.111276.

DOI:10.4103/0973-1296.111276
PMID:23772111
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3680855/
Abstract

BACKGROUND

That nimodipine (NMD) is potentially useful for ophthalmic treatment. However, the effect of NMD is unknown on retinal degenerative diseases.

OBJECTIVE

The purpose of the present study was to investigate the effect of NMD on N-methyl-N-nitrosourea (MNU)-induced retinal degeneration (RD) and elucidate its possible mechanisms.

MATERIALS AND METHODS

Morphological observation of NMD on MNU-induced RD was evaluated by light microscopy and electron microscopy. Nonenzymatic antioxidant glutathione (GSH) was measured by a colorimetric method. Transforming growth factor-beta (TGF-β) was measured by enzyme-linked immunosorbent assay (ELISA). Telomerase was detected by reverse transcriptase polymerase chain reaction (RT-PCR).

RESULTS

The significantly protective effect of NMD on MNU-induced RD was demonstrated morphologically. NMD increased the content of GSH and decreased the level of TGF-β in rat retina. RT-PCR analysis demonstrated that NMD treatment significantly decreased mRNA level of telomerase.

CONCLUSION

These data suggest that NMD inhibit MNU-induced RD in rats. The expressions of TGF-β, telomerase and GSH contents might partially contribute to its protective effects on MNU-induced RD.

摘要

背景

尼莫地平（NMD）可能对眼科治疗有用。然而，NMD对视网膜退行性疾病的作用尚不清楚。

目的

本研究旨在探讨NMD对N-甲基-N-亚硝基脲（MNU）诱导的视网膜变性（RD）的影响，并阐明其可能的机制。

材料与方法

通过光学显微镜和电子显微镜评估NMD对MNU诱导的RD的形态学观察。采用比色法测定非酶抗氧化剂谷胱甘肽（GSH）。通过酶联免疫吸附测定（ELISA）测量转化生长因子-β（TGF-β）。通过逆转录聚合酶链反应（RT-PCR）检测端粒酶。

结果

形态学证明NMD对MNU诱导的RD具有显著的保护作用。NMD增加了大鼠视网膜中GSH的含量并降低了TGF-β的水平。RT-PCR分析表明，NMD处理显著降低了端粒酶的mRNA水平。

结论

这些数据表明NMD可抑制大鼠MNU诱导的RD。TGF-β、端粒酶的表达和GSH含量可能部分有助于其对MNU诱导的RD的保护作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/837e/3680855/9e09d2a1968f/PM-9-149-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/837e/3680855/200a5a1d83ed/PM-9-149-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/837e/3680855/c94cfaccd5c6/PM-9-149-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/837e/3680855/95097fb7f52f/PM-9-149-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/837e/3680855/c180fa50c84b/PM-9-149-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/837e/3680855/9e09d2a1968f/PM-9-149-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/837e/3680855/200a5a1d83ed/PM-9-149-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/837e/3680855/c94cfaccd5c6/PM-9-149-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/837e/3680855/95097fb7f52f/PM-9-149-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/837e/3680855/c180fa50c84b/PM-9-149-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/837e/3680855/9e09d2a1968f/PM-9-149-g006.jpg

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3
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CNS Neurosci Ther. 2016 Jan;22(1):7-14. doi: 10.1111/cns.12471. Epub 2015 Oct 28.
转化生长因子-β信号传导至生长停滞、细胞凋亡及上皮-间质转化的机制。
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4
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