Reproductive Medical Center of Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
FEBS J. 2013 Aug;280(16):3920-7. doi: 10.1111/febs.12391. Epub 2013 Jul 5.
Adiponectin is an adipocyte hormone that is predominantly secreted by adipocytes, and has important roles in glucose and lipid homeostasis. Recent studies have shown that adiponectin is also involved in the regulation of many endocrine organs, such as the ovary, adrenal gland, and pituitary. However, its biological role in male testes is largely unexplored. The present findings demonstrate the presence of adeponectin receptors (adiponectin receptor 1 and adiponectin receptor 2) in TM3 cells derived from mouse Leydig cells. Proinflammatory cytokine treatment significantly downregulated mRNA and protein levels of adiponectin receptor 1 and adiponectin receptor 2. However, adiponectin pretreatment successfully inhibited the signaling pathway mediated by proinflammatory cytokines. At the molecular level, we provide compelling evidence that adeponectin achieves this by suppressing nuclear factor-κB activation through promotion of AMP-activated protein kinase phosphorylation. Thus, our data clearly indicate that adiponectin plays a protective role in Leydig cells through its anti-inflammatory actions.
脂联素是一种主要由脂肪细胞分泌的脂肪细胞激素，在葡萄糖和脂质稳态中具有重要作用。最近的研究表明，脂联素还参与许多内分泌器官的调节，如卵巢、肾上腺和垂体。然而，其在男性睾丸中的生物学作用在很大程度上尚未被探索。本研究结果表明，脂联素受体（脂联素受体 1 和脂联素受体 2）存在于源自小鼠睾丸间质细胞的 TM3 细胞中。促炎细胞因子处理显著下调了脂联素受体 1 和脂联素受体 2 的 mRNA 和蛋白水平。然而，脂联素预处理成功抑制了促炎细胞因子介导的信号通路。在分子水平上，我们提供了令人信服的证据表明，脂联素通过促进 AMP 激活的蛋白激酶磷酸化来抑制核因子-κB 的激活，从而实现这一点。因此，我们的数据清楚地表明，脂联素通过其抗炎作用在睾丸间质细胞中发挥保护作用。
