使用插入缺失标记通过定量PCR进行嵌合体检测。
Chimerism testing by quantitative PCR using Indel markers.
作者信息
Gendzekhadze Ketevan, Gaidulis Laima, Senitzer David
机构信息
HLA laboratory, City of Hope, Duarte, CA, USA.
出版信息
Methods Mol Biol. 2013;1034:221-37. doi: 10.1007/978-1-62703-493-7_11.
Engraftment monitoring is critical for patients after Hematopoietic Stem Cell Transplantation (HSCT). Complete donor chimerism is the goal; therefore, early detection of rejection and relapse is crucial for guiding the patient post HSCT treatment. Quantitative PCR for chimerism testing has been reported to be highly sensitive. In this chapter we discuss the quantitative PCR (qPCR) method using 34 Indel (Insertion and Deletion) genetic markers spread over 20 different chromosomes.
植入监测对于造血干细胞移植（HSCT）后的患者至关重要。实现完全供体嵌合是目标；因此，早期检测排斥反应和复发对于指导HSCT后患者的治疗至关重要。据报道，用于嵌合检测的定量PCR具有高度敏感性。在本章中，我们将讨论使用分布在20条不同染色体上的34个插入缺失（Indel，插入和缺失）基因标记的定量PCR（qPCR）方法。
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