Smith John D, Rose Marlene L
Transplant Immunology, Harefield Hospital, Imperial College, Harefield, UK.
Methods Mol Biol. 2013;1034:297-303. doi: 10.1007/978-1-62703-493-7_15.
The definition of HLA-specific antibodies in solid organ transplant patients is a necessary tool for recipient selection prior to transplantation and monitoring for rejection post transplant. Solid phase assays can detect both complement fixing and non-complement fixing HLA-specific antibodies. Here we describe a method for determining the presence of complement fixing HLA-specific antibodies using a sensitive solid phase assay.
确定实体器官移植患者中HLA特异性抗体的定义，是移植前受体选择以及移植后排斥反应监测的必要工具。固相分析可以检测补体结合型和非补体结合型HLA特异性抗体。在此，我们描述一种使用灵敏的固相分析来确定补体结合型HLA特异性抗体存在情况的方法。
