Jackson Annette M, Lucas Donna P, Badders Jessica L
Immunogenetics Laboratory, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Methods Mol Biol. 2013;1034:319-29. doi: 10.1007/978-1-62703-493-7_18.
Flow cytometric crossmatch tests provide a donor-specific, cell based method for the detection of alloreactive antibodies in the sera of transplant patients. Conventional crossmatch tests used in solid organ transplantation utilize lymphocytes as target cells to detect the presence of alloreactive HLA antibodies. Isolation of endothelial precursor cells (EPCs) from peripheral blood now allows testing for antibodies reactive with non-HLA endothelial cell antigens.
流式细胞术交叉配型试验提供了一种针对供体的、基于细胞的方法，用于检测移植患者血清中的同种异体反应性抗体。实体器官移植中使用的传统交叉配型试验利用淋巴细胞作为靶细胞来检测同种异体反应性HLA抗体的存在。现在，从外周血中分离内皮祖细胞（EPC）使得检测与非HLA内皮细胞抗原反应的抗体成为可能。
