Fernández-Ruiz J J, de Miguel R, Hernández M L, Ramos J A
Department of Biochemistry, Faculty of Medicine, Complutense University, Madrid, Spain.
Pharmacol Biochem Behav. 1990 Jul;36(3):603-6. doi: 10.1016/0091-3057(90)90262-g.
This paper studies the time-course of the effects of pharmacological administrations of ovarian steroids on the functional state of dopaminergic terminals in the striatum and the limbic forebrain, using the ratio between the contents of dopamine (DA) and its metabolite, L-3,4 dihydroxyphenylacetic acid (DOPAC), as an index of nerve activity. Estradiol produced an increase in the dopaminergic activity of both limbic and striatal neurons, reflected in the high DOPAC/DA ratio observed in both areas. This estrogenic effect was only observed at 4 hours, disappearing in the subsequent times studied. The effect was antagonized by progesterone in both tissues, since a single injection of this steroid to estrogen-pretreated rats restored to control values the estradiol-induced increase, suggesting the existence of negative interactions between both steroids. Furthermore, treatment with progesterone produced also a late decrease of the DOPAC/DA ratio in the striatum, which was observed only in the animals nonpretreated with estrogens.
本文利用多巴胺（DA）及其代谢产物L-3,4-二羟基苯乙酸（DOPAC）的含量之比作为神经活动指标，研究了卵巢甾体药物给药对纹状体和边缘前脑多巴胺能终末功能状态影响的时间进程。雌二醇使边缘和纹状体神经元的多巴胺能活性增加，这反映在两个区域观察到的高DOPAC/DA比值上。这种雌激素效应仅在4小时时观察到，在随后研究的时间里消失。在两个组织中，孕酮均拮抗了这种效应，因为向经雌激素预处理的大鼠单次注射这种甾体可使雌二醇诱导的增加恢复到对照值，提示两种甾体之间存在负性相互作用。此外，孕酮处理还使纹状体中的DOPAC/DA比值出现后期下降，这仅在未用雌激素预处理的动物中观察到。
