NanoScience Technology Center, University of Central Florida, Florida, USA.
Anal Chem. 2013 Jul 16;85(14):6953-7. doi: 10.1021/ac401543t. Epub 2013 Jun 27.
The activity of anticancer drugs determined under normal conditions cannot accurately reflect true drug efficacy in a patient, as a tumor is often under low oxygen (hypoxia) conditions. In addition, patient responses to the same therapy can be drastically different due to tumor heterogeneity. This paper describes the use of single cell halo assay for detection and quantification of DNA damage induced by anticancer drugs or radiation under hypoxic conditions. By combining classical halo assay and state-of-the-art microfabrication techniques, this single cell approach allows drug and radiation responses of cancer cells to be determined without population interference. The results from single cell assay indicate a diminished level of DNA damage at hypoxic conditions compared with those at normal conditions at the same drug concentrations or radiation dose, suggesting in vitro preclinical studies of drug and radiation activity can be performed under conditions that mimic physiological conditions of tumors and without population interference.
在正常条件下测定的抗癌药物活性不能准确反映患者体内的真实药物疗效，因为肿瘤通常处于低氧（缺氧）环境中。此外，由于肿瘤异质性，患者对同一治疗的反应可能有很大差异。本文描述了使用单细胞晕环测定法检测和定量缺氧条件下抗癌药物或辐射引起的 DNA 损伤。通过将经典的晕环测定法和最先进的微制造技术相结合，这种单细胞方法可以在没有群体干扰的情况下确定癌细胞对药物和辐射的反应。单细胞测定的结果表明，与相同药物浓度或辐射剂量下正常条件相比，缺氧条件下的 DNA 损伤水平降低，表明可以在模拟肿瘤生理条件下进行药物和辐射活性的体外临床前研究，而不会产生群体干扰。
