Tianjin Key Laboratory of Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Science, Tianjin 300192, China.
Radiat Oncol. 2013 Jun 19;8:146. doi: 10.1186/1748-717X-8-146.
MiRNA-21 was previously reported to be up-regulated in many kinds of cancer. In the present study, we want to investigate the potential role of miRNA-21 in non-small cell lung cancer.
Expression of miRNA-21 was detected in 60 non-small cell lung cancer (NSCLC) samples and adjacent histologically normal tissue using RT-qPCR, Correlation between miRNA-21 expression and clinicopathological features of NSCLC was analyzed using statistical software. The effect of miRNA-21 expression on the growth and apoptosis of A549 cells induced by irradiation was examined.
miRNA-21 expression increased in non-small cell lung cancer. Expression of miRNA-21 was positively associated with lymph node metastasis, clinical stage and poor prognosis. Multivariate Cox regression analysis showed that miRNA-21 was an independent prognostic factor for patients. Down-regulation of miRNA-21 inhibited proliferation and cell cycle progress of A549 cells and sensitized cells to radiation. Decreased miRNA-21 expression promoted the apoptosis of A549 cells induced by irradiation.
miRNA-21 may be considered as a potential novel target for future development of specific therapeutic interventions in NSCLC.
miRNA-21 先前被报道在多种癌症中上调。在本研究中，我们希望研究 miRNA-21 在非小细胞肺癌中的潜在作用。
使用 RT-qPCR 检测 60 例非小细胞肺癌（NSCLC）样本及其相邻组织中 miRNA-21 的表达，使用统计软件分析 miRNA-21 表达与 NSCLC 临床病理特征的相关性。检测 miRNA-21 表达对 A549 细胞照射诱导的生长和凋亡的影响。
miRNA-21 在非小细胞肺癌中表达增加。miRNA-21 的表达与淋巴结转移、临床分期和预后不良呈正相关。多因素 Cox 回归分析表明，miRNA-21 是患者的独立预后因素。下调 miRNA-21 抑制 A549 细胞的增殖和细胞周期进程，并使细胞对辐射敏感。降低 miRNA-21 的表达促进了照射诱导的 A549 细胞凋亡。
miRNA-21 可被视为 NSCLC 未来发展特异性治疗干预的潜在新靶点。
