Université de Rouen, Laboratoire de Microbiologie Signaux et Microenvironnement, EA 4312, 76821 Mont Saint Aignan, France.
Environ Pollut. 2013 Sep;180:199-205. doi: 10.1016/j.envpol.2013.05.040. Epub 2013 Jun 16.
The impact of a multiple contamination by polycyclic aromatic hydrocarbons (PAHs) was studied on permanent grassland soil, historically presenting low contamination (i.e. less than 1 mg kg(-1)). Soil microcosms were spiked at 300 mg kg(-1) with either single or a mixture of seven PAHs. While total dissipation of the phenanthrene was reached in under 90 days, only 60% of the PAH mixture were dissipated after 90 days. Interestingly, after 30 days, the abundance of the GammaProteobacteria class (assessed by qPCR) become significantly higher in microcosms spiked with the PAH mixture. In addition, the specific abundance of the cultivable Pseudomonas spp., which belong to the GammaProteobacteria class, increased earlier and transiently (after 8 days) in the microcosms spiked with the PAH mixture. Consequently, we propose to use the GammaProteobacteria as a bioindicator to detect the impact on the bacterial community of a multiple contamination by PAHs in agricultural soils.
多环芳烃（PAHs）的多重污染对历史上污染程度较低（即低于 1mg/kg）的永久性草地土壤的影响进行了研究。土壤微宇宙以 300mg/kg 的浓度添加了单一组分或 7 种 PAHs 的混合物。虽然菲在不到 90 天内完全消散，但 90 天后只有 60%的 PAH 混合物被消散。有趣的是，30 天后，用 PAH 混合物接种的微宇宙中γ变形菌（通过 qPCR 评估）的丰度显著升高。此外，属于γ变形菌的可培养假单胞菌的特定丰度在 PAH 混合物接种的微宇宙中更早且短暂（8 天后）增加。因此，我们建议使用γ变形菌作为生物指标，以检测农业土壤中 PAHs 多重污染对细菌群落的影响。
