Department of Anesthesiology, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, P.R. China.
Mol Med Rep. 2013 Aug;8(2):401-6. doi: 10.3892/mmr.2013.1530. Epub 2013 Jun 18.
Findings of previous studies have revealed that intestinal mucosal mast cells (IMMCs) are involved in small intestinal ischemia‑reperfusion injury (IIRI). However, time-course changes of mast cell counts and mast cell function in this process remain unclear. The present study aimed to observe the number of IMMCs and to investigate the correlation between their activation and small intestine injury at various time points during the period of small intestinal ischemia reperfusion (IIR). Healthy male Kunming mice were randomly divided into five groups, and were subjected to occlusion of the superior mesenteric artery (SMA) for 30 min and followed by reperfusion for 1, 3, 6 and 12 h. By contrast, the SMA was isolated but not clamped in the baseline group. Chiu's scores were assessed by light microscopy, tryptase protein and MCP7 protein expression in the intestine were quantified, and mast cell counts and levels of histamine and TNF-α in the intestine were measured. The results showed that IIR induced severe intestine injury within 12 h as demonstrated by Chiu's scores that was greatly increased as compared to the baseline group, accompanied by increased mast cell counts, histamine and TNF-α levels. However, the Chiu's scores were reduced in the IIR 12 h group compared with the IIR 1 h, IIR 3 h and IIR 6 h groups, with concomitant decreased mast cell counts, histamine and TNF-α levels. The tryptase and MCP7 protein expression was markedly increased in the IIR 1 h and IIR 3 h groups as compared with the baseline group, whereas this expression was gradually decreased at 6 and 12 h after reperfusion. The results of the present study suggest that IIR results in severe mucosal destruction within 6 h after reperfusion, associated with mast cell activation and substantial increases in the mast cell counts.
先前的研究结果表明，肠道黏膜肥大细胞（IMMCs）参与了小肠缺血再灌注损伤（IIRI）。然而，这一过程中肥大细胞计数的时程变化及其功能与小肠损伤之间的相关性尚不清楚。本研究旨在观察 IMMC 的数量，并研究在小肠缺血再灌注（IIR）期间的不同时间点，其激活与小肠损伤之间的相关性。健康雄性昆明小鼠随机分为五组，对肠系膜上动脉（SMA）进行 30 分钟的阻断，然后再灌注 1、3、6 和 12 小时。相比之下，在基础组中，SMA 被分离但未被夹闭。通过光镜评估 Chiu 评分，定量测定肠道中胰蛋白酶蛋白和 MCP7 蛋白的表达，并测定肠道中肥大细胞计数和组织胺及 TNF-α水平。结果显示，在 12 小时内，IIR 引起严重的肠道损伤，Chiu 评分明显高于基础组，同时伴有肥大细胞计数、组织胺和 TNF-α水平增加。然而，与 IIR 1 小时、3 小时和 6 小时组相比，IIR 12 小时组的 Chiu 评分降低，同时肥大细胞计数、组织胺和 TNF-α水平降低。与基础组相比，IR 1 小时和 3 小时组胰蛋白酶和 MCP7 蛋白的表达明显增加，而在再灌注 6 和 12 小时后，这种表达逐渐减少。本研究结果表明，再灌注后 6 小时内 IIR 导致严重的黏膜破坏，与肥大细胞激活和肥大细胞计数显著增加有关。
