β-磷酸三钙生物降解支架递送 rhBMP-2 和 PRP 对非贯通性兔颅骨缺损模型中新骨形成的影响。
The effect of rhBMP-2 and PRP delivery by biodegradable β-tricalcium phosphate scaffolds on new bone formation in a non-through rabbit cranial defect model.
机构信息
Department of Prosthodontics, Dental Science Research Institute and BK21 Project, School of Dentistry, Chonnam National University, 33 Yongbong-ro, Buk-gu, Gwangju, 500-757, South Korea.
出版信息
J Mater Sci Mater Med. 2013 Aug;24(8):1895-903. doi: 10.1007/s10856-013-4939-9. Epub 2013 Jun 19.
Abstract
This study evaluated whether the combination of biodegradable β-tricalcium phosphate (β-TCP) scaffolds with recombinant human bone morphogenetic protein-2 (rhBMP-2) or platelet-rich plasma (PRP) could accelerate bone formation and increase bone height using a rabbit non-through cranial bone defect model. Four non-through cylindrical bone defects with a diameter of 8-mm were surgically created on the cranium of rabbits. β-TCP scaffolds in the presence and absence of impregnated rhBMP-2 or PRP were placed into the defects. At 8 and 16 weeks after implantation, samples were dissected and fixed for analysis by microcomputed tomography and histology. Only defects with rhBMP-2 impregnated β-TCP scaffolds showed significantly enhanced bone formation compared to non-impregnated β-TCP scaffolds (P < 0.05). Although new bone was higher than adjacent bone at 8 weeks after implantation, vertical bone augmentation was not observed at 16 weeks after implantation, probably due to scaffold resorption occurring concurrently with new bone formation.
摘要
本研究通过兔颅骨非贯通性骨缺损模型评估了在可生物降解的β-磷酸三钙（β-TCP）支架中结合重组人骨形态发生蛋白-2（rhBMP-2）或富血小板血浆（PRP）是否能加速骨形成并增加骨高度。在兔颅骨上共手术制造了四个 8mm 直径的非贯通性圆柱状骨缺损，将载有或未载 rhBMP-2 或 PRP 的β-TCP 支架放入缺损处。在植入后 8 周和 16 周时，对样本进行解剖并固定，进行微计算机断层扫描和组织学分析。与未浸渍β-TCP 支架相比，仅载有 rhBMP-2 的β-TCP 支架的骨形成明显增强（P<0.05）。虽然植入后 8 周时新骨高于相邻骨，但在植入后 16 周时未观察到垂直骨增强，这可能是由于支架吸收与新骨形成同时发生。
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