State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China.
J Chem Ecol. 2013 Aug;39(8):1070-80. doi: 10.1007/s10886-013-0303-2. Epub 2013 Jun 19.
We compared food choice and the initial response to deterrent treated diet between fifth instars of Helicoverpa armigera, a polyphagous generalist pest, and Bombyx mori, an oligophagous specialist beneficial. Bombyx mori was more behaviorally sensitive to salicin than to caffeine. The relative sensitivities were reversed for H. armigera, which was tolerant to the highest levels of salicin found in natural sources but sensitive to caffeine. A single gustatory receptor neuron (GRN) in the medial styloconic sensillum of B. mori was highly sensitive to salicin and caffeine. The styloconic sensilla of H. armigera did not respond consistently to either of the bitter compounds. Phagostimulants also were tested. Myo-inositol and sucrose were detected specifically by two GRNs located in B. mori lateral styloconic sensillum, whereas, in H. armigera, sucrose was sensed by a GRN in the lateral sensillum, and myo-inositol by a GRN in the medial sensillum. Myo-inositol responsiveness in both species occurred at or below 10(-3) mM, which is far below the naturally occurring concentration of 1 mM in plants. Larval responses to specific plant secondary compounds appear to have complex determinants that may include host range, metabolic capacity, and gustatory repertoire.
我们比较了第五龄期的棉铃虫（一种多食性的一般害虫）和家蚕（一种寡食性的有益昆虫）的食物选择和对驱虫处理饮食的初始反应。家蚕对水杨苷的行为敏感性比对咖啡因的敏感性高。而棉铃虫则相反，对水杨苷的耐受性最高，但对咖啡因敏感。家蚕中一个位于中喙感觉器上的单个味觉受体神经元（GRN）对水杨苷和咖啡因都非常敏感。棉铃虫的喙感觉器对这两种苦味化合物的反应不一致。我们还测试了取食刺激物。肌醇和蔗糖被专门检测到由位于家蚕侧喙感觉器中的两个 GRN 所感知，而在棉铃虫中，蔗糖由侧感觉器中的一个 GRN 感知，肌醇由中感觉器中的一个 GRN 感知。两种昆虫的肌醇反应都发生在 10(-3) mM 或以下，远低于植物中 1 mM 的自然浓度。幼虫对特定植物次生化合物的反应似乎具有复杂的决定因素，可能包括宿主范围、代谢能力和味觉范围。
