Jin Ren-Cun, Yu Jin-Jin, Ma Chun, Yang Guang-Feng, Zhang Jue, Chen Hui, Zhang Qian-Qian, Ji Yu-Xin, Hu Bao-Lan
Department of Environmental Sciences, Hangzhou Normal University, Hangzhou, 310036, People's Republic of China,
Appl Microbiol Biotechnol. 2014 Feb;98(3):1377-88. doi: 10.1007/s00253-013-5004-9. Epub 2013 Jun 19.
In this study, the effects of both transient and long-term inorganic carbon (IC) addition on the anaerobic ammonium oxidation (ANAMMOX) process under pseudo-steady-state and substrate inhibitions were analyzed using reactor performance and measures of sludge activity. Compared with the nitrogen removal rate (NRR) of 3.42 kg N m(-3) day(-1) in the control bioreactor (ICDR) without IC, the peak NRR reached 21.0 kg N m(-3) day(-1) in the reactor (ICAR) with sufficient IC added. It was revealed that the long-term addition of bicarbonate significantly enhanced the performance of the ANAMMOX reactor. The optimum HCO3 (-)/TN ratio was considered to be 1.20, which is lower than that in normal conditions. The IC concentration affected biomass activity, and the transient addition or removal of IC to differing sludge media caused a significant loss of activity. Sufficient addition of IC alleviated the inhibition of excess substrates, while the inhibition was aggravated by the IC limitation. The half-maximal (50 %) inhibitory concentrations of substrate for the sludge were 295 mg L(-1) NO2 (-)-N and 361 mg L(-1) NH4 (+)-N with 120 mg L(-1) of fixed HCO3 (-) and 346 mg L(-1) NO2 (-)-N and 456 mg L(-1) NH4 (+)-N with unlimited IC, respectively. Changing the HCO3 (-)/TN (in milligrams per milligram) ratio resulted in the variation of ANAMMOX stoichiometric ratios. Sludge characterization parameters in the ICDR, including biomass, extracellular polymeric substances, heme C, and so on, were lower than those in ICAR. Filamentous bacteria and spherical bacteria were also observed in the reactor with limited IC.
在本研究中，利用反应器性能和污泥活性指标，分析了在准稳态和底物抑制条件下，瞬时和长期添加无机碳（IC）对厌氧氨氧化（ANAMMOX）过程的影响。与未添加IC的对照生物反应器（ICDR）中3.42 kg N m⁻³ d⁻¹的氮去除率（NRR）相比，添加充足IC的反应器（ICAR）中NRR峰值达到21.0 kg N m⁻³ d⁻¹。结果表明，长期添加碳酸氢盐显著提高了ANAMMOX反应器的性能。最佳HCO₃⁻/TN比被认为是1.20，低于正常条件下的比值。IC浓度影响生物量活性，向不同污泥介质中瞬时添加或去除IC会导致活性显著损失。充足添加IC可缓解过量底物的抑制作用，而IC限制则会加剧抑制作用。在固定HCO₃⁻为120 mg L⁻¹时，污泥对底物的半最大（50%）抑制浓度分别为295 mg L⁻¹ NO₂⁻-N和361 mg L⁻¹ NH₄⁺-N，在IC无限制时分别为346 mg L⁻¹ NO₂⁻-N和456 mg L⁻¹ NH₄⁺-N。改变HCO₃⁻/TN（毫克/毫克）比会导致ANAMMOX化学计量比发生变化。ICDR中的污泥表征参数，包括生物量、胞外聚合物、血红素C等，均低于ICAR中的参数。在IC有限的反应器中还观察到丝状菌和球形菌。
